Prevalence and molecular characterization of Mycobacterium tuberculosis resistance to aminoglycosides in the Northeast of Iran

Background and Aim : Tuberculosis is an important cause of death with an increased antibiotic resistance. Kanamycin and amikacin are among the most effective aminoglycosides for multidrug-resistant strains. We investigated the resistance to these two antibiotics in ۱۱۱ susceptible and ۹ resistant specimens.Methods : Phenotypic method was used to assess the resistance, PCR method was performed for rrs and eis genes and the results confirmed by sequencing.Results : No resistance against kanamycin and amikacin was observed in susceptible specimens and only three resistant samples had such resistance. Two had mutations at A۱۴۰۱G nucleotide, and the nucleotide change of G→A was observed at ۱۴۸۴ position in one sample. Mutations at A۵۱۴C, A۹۰۷C, G۱۳۳۲A positions were observed in one sample. eis gene sequencing revealed that one sample had mutation in promoter region at A-۱۳G position.Conclusion : The most important mutation was at nucleotide A۱۴۰۱G in rrs gene and a low prevalence of aminoglycoside resistance was observed in northeastern Iran.

کلیدواژه ها:

Tuberculosis ، Resistance ، Aminoglycosides ، MDR-TB