Investigation of antibiotic profile and biofilmogenic ability in Klebsiella pneumonia isolates with carbapenem resistance

Background and Aim : Klebsiella pneumoniae, a gram-negative bacillus, is a member of the enterobacteriaceae family that is capable of causing various infections. Infections caused by Klebsiella pneumoniae with carbapenem resistance as an important treatment problem are increasing worldwide. The aim of the present study was to investigate the antibiotic profile and the ability of biofilm production of Klebsiella pneumoniae isolates to have carbapenemase resistance in ۱۳۹۸.Methods : ۷۳ isolated Klebsiella pneumonia were collected from patients in hospitals and laboratories in Tehran province, and after confirmation of the samples with standard biochemical experiments, the samples with the enzyme method of carbapenemase enzyme were screened with the help of the phenotypic method. Then, the antibiotic profile of the obtained samples, along with the ability to form biofilm by microplite method in laboratory conditions were examined.Results : Of the ۷۳ samples, ۲۳ (۳۱%) had carbapenemase enzyme. The highest antibiotic resistance was observed with ampicillin, chloramphenicol and ciprofloxacin antibiotics, and the lowest resistance was related to gentamicin, amikacin and cotrimoxazole. Most samples were able to form biofilm.Conclusion : The present study suggests that, unfortunately, the insulin resistance of Klebsiella pneumonia is increased compared to carbapenem antibiotics, and that the samples studied have a higher resistance to antibiotics such as phosphomycin compared to previous studies. It is recommended that these isolates be evaluated for the presence of resistance genes to Colistin and phosphomycin.

کلیدواژه ها:

Klebsiella pneumoniae ، carbapenemase ، Biofilm ، Antibiogram