Nanoporous iron oxide nanoparticle: hydrothermal fabrication, human serum albumin interaction and potential antibacterial effects

Background and Aim : Nanoporous iron oxide (Fe۳O۴) nanoparticles (NIONPs) have been widely used as promising agents in biomedical applications.Methods : Herein, the NIONPs were synthesized by one-step hydrothermal method and well-characterized by FESEM and TEM investigations. Afterwards, their interaction with human serum albumin (HSA) was studied using a range of biophysical approaches, including intrinsic and extrinsic fluorescence, far and near UV- CD, and UV-Vis spectroscopic methods as well as molecular docking investigation. Also, the antibacterial effect of NIONPs was examined on pathogenic bacteria such as Staphylococcus aureus (ATCC ۲۵۹۲۳), Klebsiella penumoniae (ATCC ۳۳۸۸۳), Enterococcus faecalis (ATCC ۲۹۲۱۲) and Pseudomonas aeruginosa (ATCC ۲۷۸۵۳) standard strains.Results : The results showed the feasible fabrication of spherical-shaped NIONPs with an average diameter of around ۱۰۰ nm. Intrinsic fluorescence spectroscopy data depicted that NIONPs formed a complex with HSA by a KSV value of ۰.۰۹۲ (μg/ml)-۱. Extrinsic fluorescence, near UV-CD and UV-vis spectroscopic methods revealed that NIONPs induced some changes on the quaternary structure of HSA, whereas Tm measurement and far UV-CD spectroscopy showed some slight changes on the secondary structure of HSA even in the presence of high concentration of NIONPs. Molecular docking study disclosed that Fe۳O۴ nanoclusters with varying morphologies and dimensions could interact with different residues on the surface of HSA molecules. In addition, antibacterial assays exhibited a significant inhibition on both Gram-positive and Gram-negative pathogenic bacteria.Conclusion : In conclusion, these NPs can be used as promising antibacterial agents.

کلیدواژه ها:

spectroscopy ، docking ، HAS