The efficacy of pulsed and continuous ultrasound against Staphylococcus aureus population in chronic rhinosinusitis patients

Background and Aim : Staphylococcus aureus is one the most important bacterial pathogens involved in chronic rhinosinusitis (CRS) may result in treatment failure. Ultrasound as an alternative therapy is able to destroy bacterial population in sinus cavities and hence improving treatment efficacy.Methods : Twenty-six patients with CRS and ۲۰ healthy controls were included in this study. Clinical specimens were collected before and after ultrasound treatment. Serially diluted samples were used for bacterial isolation and DNA was extracted for bacterial detection using quantitative PCR.Results : : S. aureus was detected in ۱۴ and ۱۷ patients using phenotypic tests and qPCR respectively. However, ۶ out of ۲۰ healthy controls were also positive for S. aureus. Among the ۶ patients, ۴ of them were completely recovered after pulsed ultrasound therapy and ۹ out of ۱۱ patients showed significant reduction of S. aureus population after continuous ultrasound treatment.Conclusion : Both the pulsed and continuous ultrasound strategy have been quantitatively decreased the S. aureus population in chronic rhinosinusitis patients (p < ۰.۰۵). This was a hopeful basis for doing more studies with ultrasound therapy as an alternative option of CRS patient’s treatment.

کلیدواژه ها:

Chronic rhinosinusitis ، Ultrasound treatment ، Staphylococcus aureus ، quantitative PCR