An Effective Method for Extracting DNA From Human Hair Samples
- سال انتشار: 1400
- محل انتشار: فصلنامه گزارش های زیست فناوری کاربردی، دوره: 8، شماره: 2
- کد COI اختصاصی: JR_JABR-8-2_007
- زبان مقاله: انگلیسی
- تعداد مشاهده: 388
نویسندگان
Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Department of Biology, Payame Noor University, Mashhad, Iran
Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
چکیده
Introduction: In historical cases, mass disasters, missing person’s identification, and Archaeogenetic investigations, the bone, teeth, and hair samples are often the best and the only biological material available for DNA typing. In this study, we demonstrated an extremely effective protocol for DNA extraction of hair. Materials and Methods: Obtaining genetic information from hair samples for DNA typing involves the following steps: sterilization, DNA extraction, quality control, PCR, and profiling. After extracting aDNA (Ancient DNA) from the hair, autosomal STR analyses were performed for each sample using the AmpFℓSTR® MiniFiler™ and Identifiler™ kits. Result: Qualitative analysis of DNA by electrophoresis showed the excellent quality of the extracted samples so that the electrophoresed bands were clearly visible. Conclusions: The results demonstrated that the FDEH method is cheaper and faster than commercial kits; moreover, this new method enhances DNA recovery from hair. Because of simple protocol and high quality, this method can be utilized in Medical.کلیدواژه ها
hair, DNA Extraction, PCR, DNA Profiling, Identificationاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.