Seroepidemiological Study of Novel Coronavirus Disease (COVID-۱۹) in Tehran, Iran
- سال انتشار: 1400
- محل انتشار: فصلنامه عفونت، اپیدمیولوژی و پزشکی، دوره: 7، شماره: 2
- کد COI اختصاصی: JR_IEM-7-2_003
- زبان مقاله: انگلیسی
- تعداد مشاهده: 577
نویسندگان
Nursing Care Research Center, Iran University of Medical Sciences, Tehran, IR. Iran.
Department of Medical Laboratory Sciences, Faculty of Medicine, Babol University of Medical Sciences, Babol, IR. Iran.
Department of Medical Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR. Iran.
Department of Medical Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR. Iran.
Nursing Care Research Center, Iran University of Medical Sciences, Tehran, IR. Iran.
Department of Parasitology, School of Allied Medical Sciences. Ilam University of Medical Sciences, Ilam, Iran.
Department of Medical Microbiology and Parasitology, Faculty of Clinical Sciences, College of Health Sciences, Bayero University, PMB ۳۰۱۱, Kano-Nigeria
Department of Medical Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR. Iran.
چکیده
Backgrounds: A novel severe acute respiratory syndrome coronavirus ۲ (SARS-CoV-۲) has now spread to all countries of the world, including Iran. Although anti-SARS-CoV-۲ antibodies may be identified in patients using immunological methods with sufficient sensitivity and specificity, the conclusive diagnosis of the disease is made using the molecular RT-PCR method. A population-based seroepidemiological survey was conducted to quantify the proportion of the exposed population with SARS-CoV-۲ antibodies and evaluate whether the antibodies are a marker of total or partial immunity compared to the population that remains susceptible to the virus. Material & Methods: This cross-sectional study was conducted to investigate the seroprevalence of COVID-۱۹ in Valiasr, Sajad, and Ghaem hospitals in Tehran, the capital of Iran, from April to the end of October ۲۰۲۰. Clotted and heparinized blood specimens (۲mL) were collected from the patients. The serum and plasma were separated and stored at −۸۰ °C until use. Anti-SARS-CoV-۲ IgG and IgM antibodies were examined in the serum samples of ۱۳۷۵ in-patients admitted to the hospitals using ELISA kits. The obtained data were analyzed using SPSS software Ver.۲۲.۰ by employing statistical tests such as Chi-square and Fisher’s exact tests. A p-value < .۰۵ was considered as significant. Findings: In total, ۱۳۷۵ participants were enrolled in this study, and SARS‐CoV‐۲ antibodies were detected in ۲۹۱ patients using IgM‐IgG antibody assay. Among the seropositive patients studied, ۱۸۷ were male (۶۴.۳%), and ۱۰۴ were female (۳۵.۷%) (p< .۰۵). The mean age of the patients was ۴۹±۸.۴ years; the majority of whom (۲۷%) were in the age group of ۳۱-۴۰ years. Also, the lowest frequency of infected cases was related to the age group of ۱-۱۰ years (p < .۰۵). The seroprevalence of SARS‐CoV‐۲ IgM or IgG antibodies was determined to be ۲۱.۲%. Diabetes mellitus was the most common underlying disease among SARS‐CoV‐۲ patients [p=.۰۵; Odd Ratio=۱.۶۱(۰.۹۰-۲.۹۱)]. Conclusion: The use of conventional serological assays, such as the enzyme-linked immunoassay (ELISA), for detecting specific IgM and IgG antibodies in SARS‐CoV‐۲ patients has a high-throughput advantage while minimizing false-negative results obtained using the RT-PCR method. In this study, the seroprevalence of SARS-CoV-۲ antibodies was determined to be ۲۱%. Control of diabetes, among other influential factors, plays an important role in the management and control of COVID-۱۹.کلیدواژه ها
SARS-CoV-۲, ELISA, COVID-۱۹, Antibodies, Seroprevalence, Iran, Tehranاطلاعات بیشتر در مورد COI
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