Extracellular matrix interaction and apoptosis of the epididymal epithelium following hypothyroidism in Balb/C mouse
- سال انتشار: 1399
- محل انتشار: سومین کنفرانس بین المللی یافته های نو در مامایی، زنان، زایمان و نازایی
- کد COI اختصاصی: ICMWMI03_061
- زبان مقاله: انگلیسی
- تعداد مشاهده: 374
نویسندگان
Department of Anatomy and Cellular Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.Department of Anatomy and Cellular Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Anatomy and Cellular Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.Department of Anatomy and Cellular Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Anatomy and Cellular Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.Department of Anatomy and Cellular Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Anatomy and Cellular Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.Department of Anatomy and Cellular Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Bou-Ali Research Institute, Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Physiology, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran.Neuroscience Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran.
چکیده
The role of epididymis in post-testicular maturation of spermatozoa is to gain the potential to fertilize the ovum. It is well known that thyroid malfunction has a negative impact on male reproductive system. Hypothyroidism causes marked structural and functional changes in the ductus epididymis, as well as is able to influence extra cellular matrix (ECM) proteins secretion and cell death. This study was designed to characterize some changes induced by hypothyroidism in the mouse epididymis. ۲۰ Balb/C mice were randomly divided into control group, and hypothyroid group that was received ۰.۰۵% ۶-n-propyl-۲-thiouracil (PTU). After the confirmation of hypothyroidism, Real-Time PCR, immunohistochemistry and Periodic Acid-Schiff (PAS) staining, TUNEL assay and biochemical measurements were carried out. laminin α۵ and collagen IV mRNA levels were upregulated in the hypothyroid group compared to the control group (p< ۰.۰۵). However, no significant differences were observed in the immunoreactivity of laminin α۵ and collagen IV proteins of epididymal basement membrane (BM) between the two groups. Also, in PAS staining no significant differences were found in the intensity of BM staining between the two groups. Hypothyroidism increased markedly epididymal epithelium apoptosis (p< ۰.۰۵). In addition, hypothyroidism reduced superoxide dismutase (SOD) enzyme activity while increased malondialdehyde (MDA) level (p< ۰.۰۵). Collectively, data indicated that probably there is a relationship between alterations in the BM components and biochemical factors and increased apoptosis in the epididymal cells. These results suggest a regulatory role of thyroid hormones on the BM and structure of epididymis.کلیدواژه ها
Epididymis, Hypothyroidism, Laminin α۵, Collagen IV, Apoptosisاطلاعات بیشتر در مورد COI
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