Therapeutic potential of mesenchymal stem cells for peripheral artery disease in a rat model of hindlimb ischemia
- سال انتشار: 1400
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 24، شماره: 6
- کد COI اختصاصی: JR_IJBMS-24-6_012
- زبان مقاله: انگلیسی
- تعداد مشاهده: 213
نویسندگان
Department of Medical Physiology, Faculty of Medicine, Fayoum University, Fayoum, Egypt
Hormones Department, Medical Research Division, National Research Centre, Giza, Egypt
Department of Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt
Department of Pathology, Faculty of Medicine, Fayoum University, Fayoum, Egypt
Department of Medical Anatomy, Faculty of Medicine, Fayoum University, Fayoum, Egypt
Department of Medical Anatomy, Faculty of Medicine, Fayoum University, Fayoum, Egypt
Department of Medical Physiology, Faculty of Medicine, Fayoum University, Fayoum, Egypt
چکیده
Objective(s): Mesenchymal stem cells are viewed as the first choice in regenerative medicine. This study aimed to elucidate the influence of BM-MSCs transplantation on angiogenesis in a rat model of unilateral peripheral vascular disease. Materials and Methods: Twenty-one rats were arbitrarily allocated into three groups (۷/group). Group I: control sham-operated rats, Group II: control ischemic group: Rats were subjected to unilateral surgical ligation of the femoral artery, and Group III: ischemia group: Rats were induced as in group II, ۲۴ hr after ligation, they were intramuscularly injected with BM-MSCs. After scarification, gastrocnemius muscle gene expression of stromal cell-derived factor-۱ (SDF-۱), CXC chemokine receptor ۴ (CXCR۴), vascular endothelial growth factor receptor ۲ (VEGFR۲), von Willebrand factor (vWF), and hypoxia-inducible factor-۱α (HIF-۱α) were analyzed by quantitative real-time PCR. Muscle regeneration and angiogenesis evaluation was assessed through H&E staining of the tissue. Furthermore, Pax۳ and Pax۷ nuclear expression was immunohistochemically assessed. Results: Rats treated with BM-MSCs showed significantly raised gene expression levels of SDF-۱, CXCR۴, VEGFR۲, and vWF compared with control and ischemia groups. H&E staining of the gastrocnemius showed prominent new vessel formation. Granulation tissue within muscles of the ischemic treated group by BM-MSCs showed cells demonstrating nuclear expression of Pax۳ and Pax۷. Conclusion: BM-MSCs transplantation has an ameliorating effect on muscle ischemia through promoting angiogenesis, detected by normal muscle architecture restoration and new blood vessel formations observed by H&E, confirmed by increased gene expression levels of SDF-۱, CXCR۴, VEGFR۲, and vWF, decreased HIF-۱α gene expression, and increased myogenic Pax۷ gene expression.کلیدواژه ها
CXC chemokine receptor ۴, Mesenchymal stem cells, Peripheral vascular disease, Vascular endothelial growth factor receptor ۲, Von Willebrand factorاطلاعات بیشتر در مورد COI
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