Efficacy of SCoT and ISSR markers in assessment of genetic diversity in some Iranian pistachio (Pistacia vera L.) cultivars

  • سال انتشار: 1397
  • محل انتشار: فصلنامه پسته و سلامت، دوره: 1، شماره: 1
  • کد COI اختصاصی: JR_PHJ-1-1_007
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 372
دانلود فایل این مقاله

نویسندگان

Amin Baghizadeh

Department of Biotechnology, Instituteof Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran

Elham Dehghan

Department of Biotechnology, Graduate University of Advanced Technology, Kerman, Iran

چکیده

Background: Start Codon Targeted (SCoT) and Inter-Simple Sequence Repeat (ISSR) markers were used to appraisegenetic diversity existed in ۲۰ samples of four Iranian pistachio (Pistacia vera L.) cultivars and relative efficiencies of themarker systems were compared.Materials and Methods: ۲۰ and ۱۵ primers were used in SCoT and ISSR markers, respectively. Effective Multiplex Ratio(EMR), Marker Index (MI), Resolving Power (RP) and Polymorphic Information Content (PIC) of the primers were assessedfor the two marker systems. Cluster analysis for molecular data was performed. Principal Coordinate Analysis (PCoA) wasdone too.Results: The most of the parameters examined were found to be more suitable in ISSR system. The most remarkable resultof the current research is that cluster analysis on SCoT and ISSR data clearly discriminated the cultivars in terms of theirgenetic characterizations. There was a high similarity between dendrogram derived from ISSR marker and dendrogramderived from both markers, in spite of some observed differences.Conclusions: It is shown in the current research that there is remarkable genetic diversity among evaluated samples. SCoTand ISSR analysis produces adequate polymorphisms for DNA fingerprinting. This research reports the first application of theSCoT marker in characterization of Iranian pistachio cultivars.

کلیدواژه ها

genetic diversity, ISSR marker, Pistachio, SCoT marker

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.