The use and evaluation of Bacterial Ghost technique to produce vaccine Against Avian Pathogenic E.coli (APEC), O۲K۱ serotype

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 263

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شناسه ملی سند علمی:

IVPC07_010

تاریخ نمایه سازی: 22 اردیبهشت 1400

چکیده مقاله:

Objective:Poultry Colibacillosis is caused by different Avian Pathogenic E.coli (APEC) serotypes and is accompanied by different symptoms and commercial losses in poultry industry. Different studies were done to prevent disease such as vaccination with killed, live attenuated, recombinantand DNA vaccine. In this study a homologous vaccine component of E. coli O۲:K۱ was produced using bacterial ghosttechnique. Controlled expression of bacteriophage PhiX ۱۷۴ lysis gene E was done with transformation of bacteria by pmET۳۲b plasmids. Temperature controlled expression ofthis gene caused tunnels and holes in the E. coli O۲:K۱ cell wall, leading to loss of cytoplasmic contents. Confirmation of E. coli O۲:K۱ ghost production was done by scanning electron microscopy and determination of ۴۸ hours’ culture and colony forming units.Materials & Methods: To evaluate the efficiency of these produced ghosts as vaccine and their ability elicit cellular and humoral immune responses, ۸۵ one day old chickens from Ross ۳۰۸ breed were divided into the following ۵ groups; group ۱ (non-immunized control), group ۲ (vaccine administered by injection of E. coli O۲:K۱ killed vaccine), group ۳ (vaccine administered by injection of E. coli O۲:K۱ ghost), group ۴ (vaccine administered by inhalation of E. coli O۲:K۱ghost), and group ۵ (neither immunized, nor challenged as negative control). The three groups of ۲, ۳, and ۴ received vaccines at days ۷, ۱۴, and ۲۲. Groups ۱ to ۴ were challenged with the wild serotype at day ۳۳.Sampling was done at days ۱۴, ۲۵ and ۳۸. Evaluation of post-mortem lesions by scoring of carcasses andhumoral immune responses by measuring of IgG (IgY) and IgA were done in all groups. Results & Conclusions: Results showed that chickens injected with the bacterial ghost and the killed vaccine had the best immune response and protection compared to control groups. (P<۰/۰۵).

نویسندگان

R. H. Shahidi

Biotechnology Division, Faculty of Vet Med, Ferdowsi University of Mashhad, Mashhad, Iran

G.R Hashemitabar

Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran

M.R Bassami

Division of Poultry Diseases, Faculty of Vet Med, Ferdowsi University of Mashhad, Mashhad, Iran

H Dehghani

Biotechnology Division, Faculty of Vet Med, Ferdowsi University of Mashhad, Mashhad, Iran