Evaluation mass poropagation of Myrobalan ۲۹c rootstock in seven different culture media

Background and Aim: Myrobalan ۲۹C ( prunus ceracifera L.) belongs to Prunophora species that its origin is U.S.A. Myrobalan ۲۹c is widely used as a rootstock for apricot and plum trees due to its simplicity and compatibility to different soil conditions, so micropropagation through tissue culture is important for the production of Myrobalan ۲۹c. In this study, we compared seven basic media of MS, QL, DKW, B۵, MS Vandersaln, OLIV, WPM for proliferation of Myrobala ۲۹c rootstock. The media used supplemented with BAP (۰.۵ mg/L) + GA۳ (۱ mg/L) + IBA(۰.۰۱ mg/Land ۱۰۰ mg / l Fe-EDDHA). The results of mean comparison showed that the highest mean of main shoot length, number of leaves of main shoot, number of lateral shoot, length of largest lateral shoot, leaf number of largest lateral shoot, Wet weight, dry weight and best shoot quality were obtained respectively in media OLIV, B۵, WPM, OLIV, OLIV, B۵, B۵ and MSvandersalm (۴.۰۲, ۱۲.۹۸, ۹.۸۰, ۲.۳۷, ۷.۰۰, ۳.۳۲, ۰.۳۸ and ۵.۰۰). Also, the lowest mean of main shoot length, number of leaves of main shoots, number of lateral shoots, length of largest lateral shoot , leaf number of largest of lateral shoots, wet weight, dry weight and worst shoot quality were observed respectively in media B۵, WPM, DKW, B۵, DKW , DKW, DKW and B۵ (۲.۸۱, ۸.۷۳, ۱.۵۸, ۰.۹۲, ۴.۵, ۱۸.۱, ۱.۱۷ and ۱.۰۰). The results also showed that there was no significant difference between the highest mean number of shoots in OLIV, WPM and MS medium.Methods: Plant material The ۱۵–۲۰ cm long shoots were collected from virus-free seedlings (adult trees of) of myrobalan ۲۹C variety (apricot vegetative rootstock) which were planted in the Nethouse of Seed and Plant Certification and Registration Institute of Karaj, Iran. All the experiments were done in the tissue culture laboratory of Agricultural Biotechnology Research Institute of Iran (ABRII). Preparation and sterilization of explants The collected shoots were divided into ۳ cm pieces containing one or two lateral buds. The explants were disinfected by ۷۰% ethanol for ۱ minute, ۲.۵% nano-silver for ۱۰ minute, and ۲۵% sodium hypochlorite for ۱۰ minute, respectively. The explants were then washed for ۳ to ۵ times by rinse with sterile distilled water for ۱۰ minutes. Explants establishment Explants were cultured in jar glasses containing DKW medium supplemented with BAP (۰.۵ mg.L), IBA (۰.۰۱ mg.L), GA۳ (۱mg.L), ۱۰۰ mg.L red iron, ۳۰ g.L sucrose solid by ۵.۵ g.L agar (pH = ۵.۸). Then were transferred to a growth chamber with ۲۵۰۰-۳۰۰ lux of white-fluorescence, ۱۶.۸ D.L and ۲۴±۱ ° C. Shoot proliferation In order to proliferating shoot, the successfully established explants with ۲.۵ cm in length were transferred to MS, QL, DKW, B۵, MS Vandersaln, OLIV, WPM media supplemented with BAP (۰.۵ mg.L), IBA(۰.۰۱ mg.L), GA۳(۱ mg.L), ۱۰۰ mg.L red iron, ۳۰ g.L sucrose and solid by ۵.۵ g.L agar. After ۵ weeks, mean of main shoot length, number of leaves of main shoot, number of lateral shoot, length of largest lateral shoot, leaf number of largest lateral shoot, Wet weight, dry weight and shoot quality of per explant were measured. The experiment was conducted in a Completely Randomized Design with ۵ replicates. Each replicate was consisted of ۳ explants inside of a jar glass.Results: Mean comparison results showed that Myrobalan ۲۹c micropropagation was significantly different in seven studied media. So that the highest mean of main shoot length, number of leaves of main shoot, number of lateral shoot, length of largest lateral shoot, leaf number of largest lateral shoot, Wet weight, dry weight and best shoot quality were obtained respectively in media OLIV, B۵, WPM, OLIV, OLIV, B۵, B۵ and MSvandersalm (۴.۰۲, ۱۲.۹۸, ۹.۸۰, ۲.۳۷, ۷.۰۰, ۳.۳۲, ۰.۳۸ and ۵.۰۰). Also, the lowest mean of main shoot length, number of leaves of main shoots, number of lateral shoots, length of largest lateral shoot , leaf number of largest of lateral shoots, wet weight, dry weight and worst shoot quality were observed respectively in media B۵, WPM, DKW, B۵, DKW , DKW, DKW and B۵ (۲.۸۱, ۸.۷۳, ۱.۵۸, ۰.۹۲, ۴.۵, ۱۸.۱, ۱.۱۷ and ۱.۰۰).Conclusion: The results of this study showed that the best media for in vitro propagation Myrobalan ۲۹C were for the media OLIV, WPM and MS including BAP(۰.۵ mg/L), GA۳ (۱ mg/L) and IBA(۰.۰۱ mg/L)

کلیدواژه ها:

Tissue culture ، Myrobalan ۲۹C ، rootstock ، Micropropagation