Establishment of Iranian Physalis alkekengi hairy roots culture by Agrobacterium rhizogenes

Background and Aim: Physalis alkekengi is a valuable plant in Iran, was studied for induction of hairy roots by Agrobacterium rhizogenes. The neoplastic roots produced by A. rhizogenes infection, were characterized by high growth rate, genetic stability and producing higher levels of secondary metabolites.Methods: Hairy roots induction in this plant was investigated using AR۱۵۸۳۴ strains of A. rhizogenes and stems and leaves of P. alkekengi used as explants. PCR for rolB gene used for moleulare transformation of hairy roots.Results: Our results showed that Bacterial strain AR۱۵۸۳۴ could induce hairy root in P. alkekengi’ explants after ۷ to ۱۰ days. The frequency of transformation for leaf and stem explants were ۸۶.۹۶% and ۸۲%, respectively. Polymerase Chain Reaction (PCR) for rolB gene confirmed the morphological identification of the transformed hairy roots.Conclusion: In the current our study, we obtained P. alkekengi hairy roots that characterized with rapid growth on solid and liquid MS medium. Rapidly growing hairy roots in liquid medium is an important way for large-scale production of important secondary metabolites in P. alkekengi, such as, physalins and calystegines.

کلیدواژه ها:

P. alkekengi ، A. rhizogenes ، hairy root ، physalin and calystegine