The effect and mechanism of mirabilis antiviral protein (MAP) of four o'clock plant on CMV (Cucumber Mosaic Virus) infections on Tobacco plant

Background and Aim: In this study by using the Mirabilis antiviral protein (MAP), it was attempted to explore the MAP controlling effects' mechanism as well as its antiviral activity against the CMV under the greenhouse condition.we wanted to find out how MAP can prevent viral activity .Methods: MAP was first isolated from crude extracts of the leaves of the four o'clock plant. MAP was then cloned into a binary vector to agroinfiltrate to Nicotiana Benthamiana leaves for detecting its effects and to find out where it accumulates in cells of both inoculated N. Benthamiana with CMV and non-inoculated ones. For this aim we used laser confocal microscopy and western blot test. To understand which regions of MAP protein are responsible for apoptosis, we did mutations by altering Glutamine and Argenine to Alanine (De Virgilio et al., ۲۰۱۰. Toxins ۲, ۲۶۹۹-۲۷۳۷) to remove RIP regions abilities.Results: it was shown that after ۲ days of agroinfiltration, lesions of apoptosis emerged and in ۵ days necrosis local lesions appeared completely. we found out that without activities of ۲ regions of RIP in MAP sequence, there were no show of necrosis or apoptosis. Results indicated that accumulation of CMV is decreased by MAP treatment.Conclusion: The medicinal properties of M. jalapa have been well characterized in several studies but not much done as a plant viral diseases' remedy. A protein purified of MAP can be suggested as an antiviral protein for plant viral diseses.

کلیدواژه ها:

Ribosome inactivating protein ، morphological factor ، MAP ، Mirabilis jalapa ، vplant viruses