Identification of molecular markers associated with seed vigour index of rice using recombinant inbred line population

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 157

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شناسه ملی سند علمی:

CIGS16_437

تاریخ نمایه سازی: 14 اردیبهشت 1400

چکیده مقاله:

Background and Aim: Seed germination is one of the most critical stages in the growth and development of the plant. Identification of important genomic regions involved in the control of germination components is a main and basic step in breeding programs. Seed vigor as a germination component is an important index of seed quality and a component of seed physiological potential. Recombinant inbred lines (RILs) are powerful tools for genetic mapping.Methods: This present study aimed to investigate the seed vigour index of rice among ۱۵۴ recombinant inbred lines and the identification of microsatellite and EST-SSR markers associated with this trait. The experiment was conducted using recombination inbred line population derived cross between Shah-Pasand and IR۲۸ at University of Guilan in ۲۰۱۹. In this regard, ۱۱۰ SSR and EST-SSR markers were assessed on parents of population and identified ۴۱ polymorphic markers.Results: Five informative markers including RM۱۶۰۳۰ (on Chr.۳), RM۴۵۲ (on Chr.۲), RM۱۲۱۴۶ (on Chr. ۱), RMES۶-۱ (on Chr.۶) and RM۱۰۸۹۰ (on Chr. ۱) had a significant association with seed vigour index and explained ۱۴.۳, ۹.۹, ۹.۸, ۶.۶ and ۵.۳% of phenotype variation respectively.Conclusion: The highest coefficient of determination was estimated for RM۱۶۰۳۰. The results of the study after validation can be utilized to improve the seed vigour of rice in the breeding program.

کلیدواژه ها:

Germination components ، Marker assisted selection (MAS) ، Microsatellite markers ، QTL.

نویسندگان

Abbas Saberi Kuchesfahani

M.Sc. Student of Genetics and Plant Breeding, Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran respectively.

Atefeh Sabouri

Associate Professor of Genetics and Plant Breeding, Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran respectively.

Amin Abedi

PhD of Biotechnology , Department of Biotecnology, Facuulty of Agricultural Sciences, University of Guilan, Rasht,Iran respectively.

Ali Aalami

Associate Professor, Department of Biotecnology, Facuulty of Agricultural Sciences, University of Guilan, Rasht,Iran respectively.