Nanoliposome-encapsulated glutathione improves DNA fragmentation of frozen-thawed bull sperm
سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 198
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شناسه ملی سند علمی:
CIGS16_398
تاریخ نمایه سازی: 14 اردیبهشت 1400
چکیده مقاله:
Backgrand and Aim: The objective was to evaluate effects of liposomes for intracellular delivery and slow release of reduced glutathione (GSH) into bull sperm.Methods: Liposomes containing ۲.۵ Mm GSH were prepared using a thin-film preparation method, then sonicated to achieve different particle size (diameter of > ۵۰, ۵۰-۱۰۰ and <۱۰۰ nm, zeta potential of -۱۹ mv and polydispersity index of ۰.۲۹). In total, ۴۸ ejaculates were obtained from six Holstein bulls. Nanoliposomes containing ۲.۵ mM GSH with different particle size ( >۵۰ (NL۱), ۵۰-۱۰۰ (NL۲) or <۱۰۰ (NL۳) were added to a Tris-based extender prior to semen cryopreservation and compare to Anderomed (control).Results: Encapsulation efficiency was ۸۸.۲۴%. Total and progressive motility and membrane integrity were highest (P < ۰.۰۵) in NL۲ (۸۲.۶ ± ۰.۸ and ۴۸.۱ ± ۱.۰ respectively), but not significantly different from NL۱ (۸۵.۰ ± ۰.۸ and ۴۶.۵ ±۱.۰). Percentage of sperm with a DNA fragmentation was higher (P < ۰.۰۵) in the NL۲ group than in NL۱ and NL۳ (۴.۷ ± ۱.۰, ۵.۵ ± ۱.۰ and ۵.۹ ± ۱.۰%) but not different from Andromed (۴.۲ ± ۱.۰).Conclusion: NL۲ optimized cryosurvival of sperm from Holstein bulls.
نویسندگان
Touba Nadri
Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
Armin Towhidi
Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
Saeed Zeinoaldini
Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran