Apoptosis Inducing Effect Of Spiroaminopyrimidine Analogue In NB۴ Leukemia Cells Via Down-Regulation Of BIRC۵ Expression

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 221

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شناسه ملی سند علمی:

CIGS16_349

تاریخ نمایه سازی: 14 اردیبهشت 1400

چکیده مقاله:

Background and Aim: It has been reported that spiroaminopyrimidine derivatives inhibited the growth and proliferation of various cancer cell lines. In the present study, we evaluated cytotoxic and apoptosis-inducing effects of ۲,۴-diamino-۱,۳-diazaspiro[۵.۵]-۹-tert-butyl-۲, ۴-diene-۵-carbonitril (۹-tBAP) on NB۴ acute promyelocytic leukemia (APL) cells.Methods: The cells were treated with ۱۰–۱۰۰ µM of ۹-tBAP. Cytotoxic activity of the compound was measured using the MTT assay. Apoptosis was investigated by Hoechst ۳۳۲۵۸ staining as well as by Annexin V/PI double staining.Results: The compound under study was found to be highly active cell growth inhibitor with IC۵۰ of ۳۰.۰ ± ۳.۵ µM inducing apoptosis in NB۴ cells. Cell cycle analysis by flow cytometry showed a time-dependent increase in sub-G۱ cell population. Real-time polymerase chain reaction analysis revealed that the treatment with the compound down-regulated the BIRC۵ expression in a time-dependent manner.Conclusion: ۹-tBAP displayed potent anti- leukemic activity in vitro thus warranting further investigation.

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نویسندگان

MohammadJavad DehghanNayeri

Department of biology,Faculty of natural science,University of tabriz,Tabriz,Iran

Majid Mahdavi

Department of biology,Faculty of natural science,University of tabriz,Tabriz,Iran