Investigation of CD۴۴ (C۳۵۳۶۴۷G and -G۱۸۷۱۱۶A) Gene Polymorphisms in the Benign Prostatic Hyperplasia: A Case-control Study

Background and Aim: CD۴۴ is a cell surface glycoprotein, which is known as a cell adhesion molecule/CAM. It plays a major role in cell-cell adhesion, cell-substrate interaction, lymphocyte homing, idiopathic recurrent miscarriage, and tumor metastasis. On the other hand, malignant cells can initiate a tumor metastasis by sustaining proliferative signaling. Thus it is probable that some of the CD۴۴ gene mutations can stimulate and sustain progression of cells through their growth and division cycles. The aim of this study is to investigate CD۴۴-C۳۵۳۶۴۷G and -G۱۸۷۱۱۶A gene polymorphisms with benign prostatic hyperplasia by RFLP.Methods: The boiling DNA extraction performed from ۱۵۰ patients and ۷۰ normal control blood samples, which collected from Rouhani Hospital (Babol, Iran). The PCR- RFLP have been used from products of the C۳۵۳۶۴۷G (۴۰۱ bp) and G۱۸۷۱۱۶A (۶۹۴ bp), and digested by HinfI and HpaII restriction enzymes pattern, respectively. For visualizing the RFLP bonds, the ۱% agarose gel electrophoresis has been used.Results: There are ۳ types of bonds in C۳۵۳۶۴۷G genotyping with ۱۲۱, ۲۸۰ bp; ۴۰۱, ۱۲۱, ۲۸۰ bp; and ۴۰۱ for CC; CG; and GG genotypes, respectively. Also, we observed ۳ types of bonds in G۱۸۷۱۱۶A genotyping with ۴۵۳, ۲۴۱ bp; ۶۹۴, ۴۵۳, ۲۴۱ bp; and ۶۹۴ for GG; GA; and AA genotypes, respectively. The frequency of alleles was almost significantly different (Pvalue = ۰.۰۹) in case and control groups.Conclusion: In spite of what our study showed, the frequency of allele might be more different in case and control groups if the number of sample increases. And so, these intron-SNPs of the CD۴۴ as a tumor marker on the stability of transcript molecules; can be studied by other researchers.

کلیدواژه ها:

Benign Prostatic Hyperplasia ، Recurrent miscarriage ، CD۴۴ gene ، in silico ، Transduction pathways