Evaluating toxic effects of biosynthetic nanocurcumin on human gastric cancer cells

Background and Aim: Gastric cancer is ranked as the fifth most frequently diagnosed cancer and the third for mortality in the world. Despite chemotherapy is a common therapeutic option for gastric cancer patients, efficacy of current regimens is still low. To introduce a novel nanoparticle with cytotoxic activity against human gastric cancer cells, we evaluated effects of biosynthetic nanocurcumin derived from a gram positive coccus.Methods: For biosynthesis of nanocurcumin, whole bacterial cell cultivated for ۲۴ h in the presence of curcumin was used, followed by centrifugation at ۵۰۰۰ rpm. To prepare different concentrations of nanocurcumin, as well as curcumin itself, DMSO was used as solvent, and final concentrations were made by DMEM supplemented with ۱۰% FBS just before each experiment. Then, MKN۴۵ cells, a human gastric cancer cell line, were treated with ۵, ۱۰, ۲۰ and ۴۰ µg/ml of each agent for ۲۴ h. At the end, cell viability was determined by alamar blue as a colorimetric assay, and changes in the cell morphology were recorded by an inverted microscope.Results: Assessment of MKN۴۵ cell viability revealed that ۹۲%, ۶۶%, ۳۴% and ۳۵% of cells were alive after administration of ۵, ۱۰, ۲۰ and ۴۰ µg/ml nanocurcumin, respectively. Moreover, viability of cells were calculated as ۶۹%, ۴۷%, ۴۳% and ۲۵% when curcumin was used in concentrations of ۵, ۱۰, ۲۰ and ۴۰ µg/ml, respectively.Conclusion: Based on present findings, which indicated toxic effects of nanocurcumin against gastric cancer cells, synthesis of nanocurcumin by microorganisms could be considered as reliable and eco-friendly method to obtain novel anticancer agents.

کلیدواژه ها:

Gastric cancer ، Nanocurcumin ، Biosynthesis ، Toxic effects