Molecular Diagnostics of Candida albicanse Secreted Phospholipase B Genes, Yeast, Popular Vaginal Infectious Agent in Women in Tehran City

Background and Aim: Phospholipases are functional enzymes that are important in the biological process of organisms. These enzymes can hydrolyze glycophospholipids with esterase activity and they invade tissue and have an important role in pathogenesis. Candida albicans is one of the most common microorganisms in the human body that affected by phospholipase enzymes. The aim of this study was molecular diagnosis of C. albicans genes plb۱ and plb۲, and vaginal infection agent in women in Tehran CityMethods: Fifty of C. albicans isolates were collected from patients with vaginitis symptoms who had referred to health centers in Tehran city. DNA extraction was performed using a DNA extraction Kit and amplified by PCR for detecting plb genes (plb۱ and plb۲) with specific primers. The PCR products were sequenced by Microsynth Company in Switzerland and FASTA format of sequences were extracted using Chromas Lite software. Then sequence analysis was performed by BLAST program in NCBI database. DNA sequences translated to amino acid sequences using sequence translation tools (EBI, (EXPASY). The three-dimensional structure and the secondary and estimated protein structure were determined by PHYER۲ Protein Fold Recognition Server.Results: Results of homology comparison C. albicans gene plb۱ shows ۸۸.۷۱% homologous with C. albicans plb۱ gene for phospholipase B۱ from USA (Muzzey et al., ۲۰۱۸). The secondary structure of the protein showed that the protein structure of this gene contains ۳% alpha helix and ۴۸% beta strand. Maximum phylogenetic relationship with the Candida albicans TIMM ۱۷۶۸. Molecular analysis of the PLB۲ gene shows ۸۸.۳۳% homology similarity with C. albicans plb۲ gene for phospholipase B۲ from USA (Hoover et al., ۱۹۹۸). Secondary structure of protein of C. albicans gene plb۲ contains ۵۶% alpha helix and ۶% beta strand structure. And maximum likelihood (phylogenetic relationship) with the Candida albicans SC۵۳۱۴.Conclusion: Candida albicanse secreted phospholipase B genes (plb۱, plb۲) polymorphism were found in isolates from patients with vaginitis symptoms who had referred to health centers in Tehran.

کلیدواژه ها:

Candidiasis ، Candida albicans ، Phospholipase B Genes ، Vaginal ، Infection ، Yeast.