Investigation of relationship between changes in expression of oncogene LINKA in acute lymphoblastic Leukemia cell line Jurkat E۶.۱ under treatment with ۶MP chemotherapy drug.

Background and Aim: Leukemia is characterized by the excessive growth of blood cells that affects the bone marrow. It can also be seen in adults, especially people over ۶۵ years. The aim of this study was to evaluate changes in the expression of AKT\PI۳K signaling pathway LINKA gene in acute lymphoblastic leukemia cell line Jurkat E۶.۱ under treatment with ۶MP.Methods: ۶MP was prepared at different concentrations (۱۰,۲۵ macro molar).The Jurkat E۶.۱ cancer cells were treated with chemotherapy after the cell passage in (۲۴ hours). RNA extraction and Cdna synthesis were performed and the LINKA gene expression was evaluated by Real Time PCR. The results were analyzed by REST Relative REST analaysis softwere.Results: The results of this study showed that LINKA gene in concentrations (۱۰ and ۲۵ macro molar) in ۲۴ hours showed a significant decrease (p<۰.۰۰۱) from the expression level of the gene.Conclusion: LINKA gene expression changes are influenced by the ۶MP drug and decrease the expression of this oncogene in all concentrations and time, depending on time and concentration. The results of this study can be used to control and treat the optimal treatment of patients with leukemia, by identifying molecular pathways in the function of the chemotherapy drugs used, as well as introducing new drugs and preventing untreated control of JURKAT cancer cells.

کلیدواژه ها:

Acute lymphoblastic leukemia ، LINKA ، ۶MP ، Jurkat E۶.۱