Knock down of SIRLNT by Antisense LNA GapmeRs and evaluation of its inhibitory effect on apoptosis and necrosis in human breast cancer cells

Background and Aim: long non-coding RNAs (lncRNAs) play a critical role in progression of human disease especially in cancer. lncRNAs could be as either oncogenes or tumor suppressor genes. In the current study, we targeted oncogenic lncRNA SIRLNT (SIRT۱ regulating lncRNA tumor promoter) as one of the most significantly up-regulated lncRNA in human breast cancer.Methods: MCF-۷ cell line was transfected with lncRNA-SIRLNT antisense LNA GapmeRs at different time points. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to evaluate expression of lncRNA SIRLNT. Annexin V Apoptosis Detection Kit was used for apoptosis and necrosis analysis.Results: The results indicated lncRNA SIRLNT was significantly downregulated after transfection of MCF-۷ cell line by Antisense LNA GapmeR. Significantly, we found that the LncRNA SIRLNT knockdown lead to increase of apoptosis and necrosis of MCF-۷ cell line.Conclusion: Our finding suggested that lncRNA SIRLNT inhibition could be a promising therapeutic strategy to prevent progression of breast cancer.

کلیدواژه ها:

Breast cancer ، LncRNA SIRLNT ، LNA GapmeRs oligonucleotides ، MCF-۷