Studying the expression of HOTAIR in blood samples of high grade colorectal cancer patients compared with normal people

Background and Aim: Colorectal cancer (CRC), one of the most common malignant human tumors, has a high mortality and is the second leading cause of cancer death in the world. Biomarkers that enable the detection of CRC at an early stage, can improve prognosis, prediction of treatment response, and risk of recurrence. The ability to detect biomarkers in patient blood samples provides the most practical screening tool for CRC, and the detection of biomarkers in blood or other biological fluids provides many advantages, including minimal invasion and easy access. HOTAIR as a candidate lncRNA has important regulatory roles in the development and progression of cancer and will be a new potential target in tumor therapy. HOTAIR LncRNA, because of its rol in different cellular pathways have been considered in recent years. CRC-related lncRNAs could enter into the circulation, and their differentiated expression in blood could be used as diagnostic biomarkers for CRC. Based on previous studies of increased expression of HOTAIR in colorectal cancer tumor tissues as well as the study of this non-coding RNA in the blood of colorectal cancer patients in Central Europe and its increased expression in patients, we concluded that evaluate HOTAIR gene expression in the blood of patients with colorectal cancer for the first time in Iran.Methods: In this study, blood samples were collected from ۱۵ high grade colorectal cancer patients and ۱۵ healthy individuals. Peripheral blood mononuclear cells were isolated using ficoll and total RNA was extracted using RNX-Plus. After quantitative and qualitative analysis of the extracted RNA, cDNA was synthesized and Real-time PCR technique was used to evaluate the expression of the studied genes.Results: In this study, it was found that HOTAIR gene expression in blood samples of patients with high grade colorectal cancer was significantly increased ۷.۸ times compared to healthy individuals.Conclusion: This study may partly illustrate the role of the HOTAIR gene in the tumorigenesis process and may serve as a pilot study for further investigation of this gene and its potential role as a potential biomarker or therapeutic target.

کلیدواژه ها:

Colorectal cancer ، lncRNA ، HOTAIR ، biomarker