Evaluation of the expression of RSBN۱L-AS۱ Long non-coding RNA in breast cancer and its relationship with clinicopathological information and reproductive factors

Background and Aim: Accumulating evidence has been reported that long noncoding RNAs (lncRNAs) play important roles in many types of disease through competing endogenous RNA (ceRNA) mechanism. Recently, an oncogenic lncRNA named RSBN۱L-AS۱ has been recognized. Though, its dysregulation and function in breast cancer (BC) is uncertain yet. The purpose of this study is to examine the RSBN۱L-AS۱ expression and its roles in BC.Methods: The expression level of lncRNA RSBN۱L-AS۱ was screened using real-time PCR. Also we applied bioinformatics tools to assess the RSBN۱L-AS۱-microRNAs-mRNAs network in BC with focus on ErbB signaling pathway.Results: lncRNA RSBN۱L-AS۱ expression in breast tumors was upregulated relative to that in breast margins. More ever, it was found that RSBN۱L-AS۱ expression increased associated with higher tumor size. Bioinformatics analysis shows that RSBN۱L-AS۱ is a microRNA (miRNA) sponge which could target cell proliferation by dysregulating of ErbB signaling pathway.Conclusion: The present results revealed that RSBN۱L-AS۱ expression is significantly upregulated in breast tumors. Also, clinicopathological information show that RSBN۱L-AS۱ expression is associated with large tumor size. LncRNA RSBN۱L-AS۱ could affect cell proliferation through dysregulating of ErbB signaling pathway by miRNAs sponge.

کلیدواژه ها:

Breast cancer ، lncRNA ، RSBN۱L-AS۱ ، ceRNA