Bioinformatic Analysis of aflR sequences belonging to some Aspergillus species

Background and Aim: The genus Aspergillus is one of Trichocomaceae members. Its species are one of the most abundant fungi in the environment which have high saprophytic capability. The genus has more than ۱۰۰ species which can grow on agricultural products and different foods. The aflatoxins are one of the most important mycotoxins which secrete to Aspergilli substrates.This toxins leads to acute and chronic aflatoxicosis on humans and animals. Due to its ability to bind to DNA and hepatocyte mitichondria, causes significant mutagenesis and carcinogenesis.Methods: In this survey for comparison of aflR sequences belonging to different aflatoxigenic Aspergillus species, many sequences were extracted from NCBI and subjected to dendrogram drawing by MEGA۷ by neighbor joining method with ۱۰۰۰ bootstrop.Results: Extracted sequences belonged to A.sojae, A.flavus , A.oryzae , A.parasiticus and A. minisclerotigenes. The results showed sequences belonging to one species but from different sources had close similarity together. A.flavus sequences had nearest similarity together as main sources of aflatoxin production among all Aspergilli. Another near clades were A.sojae and A.parasiticus, but far from A.flavus and A.oryzae sequences had intermediate position in drawn dendrogram .Conclusion: Translation of surveyed sequences to their related amino acids and products comparison may be an interesting area for next studies. The A.parasiticus strain BN۹-Ku۷۰ esdC gene used as outgroup for tree drawing.

کلیدواژه ها:

Bioinformatic ، Aspergillus ، aflR sequences ، Aflatoxin