In silico analysis of key genes and signaling pathways targeted by a panel of signature microRNAs in Hodgkin lymphoma

Background and Aim: Hodgkin's lymphoma cancer is type of lymphatic system cancer and the most common cancer diagnosed in teenagers ages ۱۵ to ۱۹ years.A recent study has suggested that microRNAs play an important role in Hodgkin's lymphoma cancer. Many investigating has been performed to identify a common molecular biomarker for use in early diagnosis and prognosis of the diseases For this purpose microRNAs recently Appearance as a good non-invasive biomarker for cancer.MicroRNAs play an important role in the regulation of gene expression in the post-transcriptional level by targeting mRNA ۳′ untranslated region (۳′ UTR) and usually downregulate its expression. The mature miRNA has a ۶–۸ nucleotide region that is recognized as the ‘seed region’ which Binds to the ۳′ UTR of target mRNA. In the present study We selected eight over expressed miR as an oncomiR for investigation of their common target genes and pathway analysis. Some bioinformatics tools were utilized to identify differentially expressed genes (DEGs) in Hodgkin's diseaseMethods: Some bioinformatics tools were utilized to identify differentially expressed genes (DEGs) in Hodgkin's disease.String and Cytoscape tools were performed for analysis of the protein-protein interaction (PPI) network construction and selection of hub genes.afterwards genes survey was conducted by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis using the Database for Annotation, Visualization and Integrated Discovery (DAVID)tool.Results: Top۱۰ key hub genes from ۳۶۶ sheared target genes were identified between eight microRNA in Hodgkin's disease. Further top ten genes from TGF-beta signaling pathway, Phosphatidylinositol signaling system, MAPK signaling pathway, ErbB signaling pathway, AMPK signaling pathway, and PI۳K-Akt signaling pathway was extracted.Conclusion: Finally, the importance of this hub genes in cellular and functional pathways in Hodgkin's disease was clarified and indicating their potential as candidate biomarkers in Hodgkin's lymphoma.