Evaluation of molecular diversity of rice varieties in Central and West Asia using microsatellite markers

Background and Aim: Rice is the most important food source for more than half of the world's population. One of the most important applications of molecular markers in plant breeding is to evaluate the potential of hereditary stocks that can provide valuable genotypes for breeding appropriate crosses. The purpose of this study was to evaluate the genetic diversity within a diverse collection of rice (Oryza sativa L.) accessions.Methods: In this study, ۶۰ microsatellite markers were used in ۶۳ rice genotypes of Central and West Asia to group rice cultivars.Results: Based on data from ۶۰ markers, it was observed that a total of ۲۵۲ polymorphic alleles were amplified with an average of ۴.۲ alleles per primer. The lowest number of alleles belonged to the RM۴۹۰ marker with ۲ alleles and the highest number was belonged to the RM۲۲۵ marker with ۸ alleles, RM۴۲۴ and RM۲۴۶ markers with ۷ alleles. The mean number of effective alleles was ۳.۷۸ which RM۴۹۰ and RM۵۴۲۳ markers had the lowest and the RM۲۲۵ and RM۲۴۶ markers had the highest value for this index. Gene diversity and amount of polymorphic information content showed that RM۲۳ and RM۲۱۲ markers had the highest value and RM۳ marker had the lowest value for these two indices. Classification of genotypes was performed by cluster analysis using PAST software. Genotypes were classified into ۵ clusters using Dice similarity coefficient and UPGMA method.Conclusion: Based on the results of cluster analysis and genetic distances, genotypes of Iran, Afghanistan and Azerbaijan can be used for hybrid production in order to increase grain yield and quality.

کلیدواژه ها:

Rice ، Diversity ، Microsatellite ، Genetic Distance.