Evaluation of Genetic Diversity in Different Basil Lines (Ocimum basilicum L.)

Background and Aim: This experiment was conducted to investigate the genetic diversity and the degree of kinship relationship among different basil lines using ISSR markers.Methods: For this experiment, ۴۵ different basil linens (including indigenous Iranian and Swiss and American native) were used. First, DNA of plant samples was extracted by CTAB method. Then, the quantity and quality of the extracted samples were investigated by spectrophotometer and horizontal gel electrophoresis. Then ۹ ISSR markers were selected and used in polymerase chain reaction (PCR) to amplify DNA fragments. Finally, the amplified DNA samples were transferred to ethidium bromide solution on a gel electrophoresis apparatus. Finally we moved to the gel duck machine for shooting. The bands were then scored according to the photographs taken from different samples.Results: In this study, according to the bands obtained from ISSR marker system, ۱۰۱ gene loci were examined, ۶۴ of which were polymorphic. This marker system produced ۶۵.۵% polymorphism and, on average, seven polymorphic gene loci. The results of the PIC statistics of each primer also showed that the highest content of polymorphic information in ISSR marker system belonged to primer five and the lowest value belonged to primer two with values of ۰.۳۴ and ۰.۱۵, respectively. The mean polymorphism information in the ISSR marker system was ۰.۲۲. The results of the MI statistic of each ISSR marker also showed that the highest MI belonged to primer ۱۰ and the lowest amount to two primers with values of ۴.۷ and ۱.۵۵, respectively. Also, the mean MI information in the ISSR marker system was ۳.۰۵.Conclusion: Genetic variation analysis in ۴۵ different lines of basil showed that, Isfahan ۲۳۱, Varamin ۱۲, Green-Birjand ۱۱, Malayer ۳۲ had the most similarity to each other, while Isfahan ۴۳۲ and Green-Birjand ۲۱ has the least amount of similarity to each other as well as to others. The relatively low level of polymorphism was obtained between different basil lines using ISSR marker systems. The similarity coefficients of the ISSR marker were ۰.۶۴ to ۰.۹۹, respectively, and ۶۵% of the polymorphisms were typically observed among populations.

کلیدواژه ها:

Basil ، ISSR ، Molecular Diversity ، PCR.