Knockdown of long non coding RNA of LINK-A suggests a potential oncogenic function in a۵۴۹ non-small cell lung carcinoma

Background and Aim: Long non coding RNAs (lncRNA) play an important regulatory roles in various pathways which dysregulation of them impact on cancer. LINK-A (long intergenic noncoding RNA for kinase activation) is an oncogenic cytoplasmic noncoding RNA with approximately ۱.۵ kbp length. LINK-A is involved in a pathway, which ultimately leads to hyperactivation of HIF۱α in normoxic condition in triple negative breast cancer. Oncogenic function of LINK-A was indicated in ovarian cancer and in human glioma cells, but its function in most of cancers is still unclear. In this study we were going to functionalize LINK-A in a۵۴۹ cell line as a model of non-small cell lung cancer.Methods: Expression of LINK-A was detected in a۵۴۹ cell line by RT-PCR and confirmed by cloning in TA-vector and sequencing. Two specific siRNAs were used to downregulation of LINK-A, along the experiment, we used Hs cell death control siRNA as a positive control and scrambled siRNA as a negative control. Consequences of LINK-A downregulation was examined by MTT, colony formation, and wound healing assay and also cell cycle analysis, annexin V/PI, Acridin orange and ethidium bromide staining were conducted to examine occurrence of apoptosis. qPCR was used to determine RNA expression change of LINK-A and some other genes downstream of HIF۱.Results: Our results revealed reduced cell viability and colony formation ability influenced by LINK-A silencing. We also observed the induction of apoptosis and declined ۲-D cell mobility, following by LINK-A downregulation.Conclusion: our findings suggest a potential oncogenic role for LINK-A in a۵۴۹ cell line as a representative for non-small cell lung cancer for the first time.

کلیدواژه ها:

LINK-A ، Long non coding RNA ، non small cell lung cancer ، a۵۴۹ cell line