یعقوب مهری الوار - Dept. of Exercise Physiology, Shahid Rajaee Teacher Training University, Tehran, Iran
علیرضا رمضانی - Dept. of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
عباسعلی گاینی - Dept. of Exercise Physiology, Shahid Rajaee Teacher Training University, Tehran, Iran
فرشته گلاب - Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
ریاض غیرتمند - Dept. of Cardiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
محمود براتی - Dept. of Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran

Background and Objective: Myocardial infraction can lead to loss of heart muscle cell, changes in ventricular function and structure, scar formation as well as leading the heart to ultimate stroke and its loss. This study aimed to investigate the effect of eight weeks of High-Intensity Interval Training (HIIT) on the gene expression of angiogenesis factors in ischemic rats. Materials and Methods: In this study, ۲۸ male Wistar rats (۲۰۰-۲۵۰ g) were randomly assigned to ۴ groups: sham, ischemia, exercise, and exercise-ischemia. Myocardial infarction (MI) was achieved via closure of LAD artery for ۳۰ minutes. A treadmill exercise program was performed by the rats for ۸ weeks, ۴۰ minutes daily, ۳ days a week. Results: The results showed that there is a significant difference between the four groups in gene expression levels of VEGF, apelin and apelin receptor (p = ۰.۰۰۱). Results also showed that the gene expression level of VEGF, apelin and apelin receptor was significantly increased in the exercise-ischemia group compared to sham (p = ۰.۰۴), ischemia (p = ۰.۰۰۲) and exercise (p = ۰.۰۰۹) groups. Results showed that exercise during ischemia can lead to an increase in VEGF gene expression but not in exercise group alone. On the other hand, a significant increase was observed in gene expression level of apelin following ischemic intervention compared to the sham group (p = ۰.۰۲۵). No difference was observed between the interval training group and sham group (p > ۰.۰۵). Meanwhile, a significant reduction in the gene expression level of apelin receptor was seen following ischemic intervention compared to the sham group (p = ۰.۰۴). Conclusion: The physiological adaptations caused by interval training are associated with different gene expressions. A regular interval training program leads to the increase of factors involved in angiogenesis and the regeneration of cardiac muscle vessels following myocardial ischemia.

Keywords: Myocardial ischemic, Angiogenesis, Intensity interval training, Apelin