Association of G۱۵۶۳۱T, CYP۲B۶ Gene Polymorphism with Susceptibility to Acute Myeloid Leukemia

Background and Objective: Acute myeloid leukemia (AML) is a clonal proliferation of immature myeloid progenitors in the bone marrow. Multiple genetic and environmental factors have been reported to be involved in the pathogenesis of AML. Cytochromes, such as cytochrome P۴۵۰ are among detoxifying enzymes whose aberrant expression can make individuals susceptible to a variety of cancers and leukemias including AML. The aim of this study was to evaluate the G۱۵۶۳۱T, CYP۲B۶ subfamily of cytochrome P۴۵۰ gene with susceptibility to AML in some AML patients and normal individuals. Materials and Method: We enrolled ۷۶ AML patients and ۸۰ normal individuals as the control group for the study of G۱۵۶۳۱ T polymorphism of CYP۲B۶ gene. This polymorphism was amplified by PCR using specific primers. The PCR products were digested by Bsr I enzyme and Electrophoresed on Agarose gel. Results: The frequency of G۱۵۶۳۱T polymorphism was significantly higher in patients (۵۵%) than the control group (۳۱%). The risk of AML (with CI = ۹۵%) in patients with GT genotype was ۱.۹۵ fold more than individuals with the same genotype in the control group. The TT genotype was found in ۴ patients with AML, but in none of the individuals in control group. Conclusion: The results show that there is a direct relationship between G۱۵۶۳۱T, CYP۲B۶ gene polymorphism and susceptibility to acute myeloid leukemia in some patients in our study population. However, further studies on larger populations are recommended.