Determination of Human Blood Index in Anopheles maculipennis and Culex theileri (Diptera: Culicidae) by Sandwich Enzyme Linked Immunosorbent Assay in Zanjan Province in ۲۰۰۳
- سال انتشار: 1383
- محل انتشار: مجله علمی پژوهشی دانشگاه علوم پزشکی زنجان، دوره: 12، شماره: 49
- کد COI اختصاصی: JR_ZUMS-12-49_008
- زبان مقاله: انگلیسی
- تعداد مشاهده: 214
نویسندگان
چکیده
Background: Mosquitoes are the most important blood feeding insects that can transmit serious diseases such as malaria and arboviruses. Each species of mosquitoes selects a specific host according to biological conditions of the region and genetic characteristics. Anopheles maculipennis complex and Culex theileri are dominant mosquito species in Zanjan. This study was carried out in order to determine the blood feeding index of mosquitoes by Sanduwich Enzyme Linked Immunosorbent Assay (ELISA) in ۲۰۰۴. Materials and Methods: An. maculipennis complex and Cx. theileri mosquitoes were collected from indoors and outdoors by pyrethrum spray catch (PSC) and light trap in ۲۰۰۳ and blood fed samples were separated and kept at -۲۰°c subsequent to drying. The samples were tested for human blood after establishing Sandwich ELISA technique. The results were analyzed through test. Results: A total of ۶۴۳۶ samples were studied, out of which ۳۰۷۲ (۴۷.۷۳%) were An. maculipennis and ۳۳۶۴ (۵۲.۳%) Cx. theileri. Human blood-feeding index in An. maculipennis complex was ۳.۲% while this rate reached ۱.۵% in Cx. theileri. An. maculipennis complex showed ۲.۱۲% more anthropophilic behavior than Cx. theileri. Anthropophilic index of this anopheles by PSC was ۰.۲%. This index was ۷.۳% (۹۳) in samples collected by light trap. There was a significant difference in two rates (p=۰۰۰۱). In PSC samples anthropophilic index was ۳۵.۱۷ times less than light trap samples. Anthropophilic index of this Culex was ۱.۸% through PSC and ۱.۳% through light trap, which shows no significant difference. Conclusion: With regard to high human blood feeding index of An. maculipennis complex, we suggest that health officials of the province pay careful attention to malaria control and monitoring programs. To reduce human blood feeding index people should be encouraged to use personal protection and keep livestock to divert mosquitoes. More research associated with molecular biology, ecology and bionomy is recommended to determine this difference.کلیدواژه ها
KEY WORDS: Anopheles maculipennis, Culex theileri, Human blood feeding, ELISA, Zanjanاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.