In vitro and in vivo toxicity and histopathological evaluation of Gd(III)anionic Linear globular dendrimer second-generation G۲-C۵۹۵ nanoprobe
- سال انتشار: 1399
- محل انتشار: مجله علوم نانو، دوره: 7، شماره: 4
- کد COI اختصاصی: JR_NAMJ-7-4_004
- زبان مقاله: انگلیسی
- تعداد مشاهده: 384
نویسندگان
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Cancer Institute Research Center, Tehran University of Medical Sciences, Tehran, Iran
Department of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
Department of Radiopharmacy, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Objective(s): Toxico-histopathological studies are used to assess the toxic impacts of nanoparticles in organism exposure. The present study aimed to evaluate the prospective nano-cytotoxicity impacts of Gd(III)-anionic linear globular dendrimer second-generation G۲-C۵۹۵ (Gd[III] dendrimer G۲-C۵۹۵) contrast nanoprobe in terms of the exposure of many nude mice organs and organisms. In addition, we assessed the potential of the Gd(III)-dendrimer G۲-C۵۹۵ nanoprobe as a novel magnetic resonance imaging (MRI) nano-contrast agent for the human breast cancer cell line (MCF-۷) and human embryonic kidney cell line (HEK-۲۹۳).Materials and Methods: Gadolinium (Gd[III]) was loaded with dendrimer G۲ and conjugated with the C۵۹۵ monoclonal antibody to generate the Gd(III)-dendrimer G۲-C۵۹۵ to determine the impact on MUC۱ beneficial cancer tumors. The cytotoxic effects of the Gd(III)-dendrimer G۲-C۵۹۵ nanoprobe on the HEK-۲۹۳ cells were also investigated in-vitro and in-vivo. In addition, the Gd(III)-dendrimer G۲-C۵۹۵ nanoprobe was used on nude mice bearing the MCF-۷ tumors to explore its specific activity against the in-vivo model of cancer.Results: The Gd(III)-dendrimer G۲-C۵۹۵ contrast nanoprobes affected the cytotoxicity of MCF-۷, and no in-vivo toxicity was induced in the HEK-۲۹۳ cells, kidneys, heart, lungs, brain, liver tissues, and other organs.Conclusion: According to the results, the Gd(III)-dendrimer G۲ and Gd(III)-dendrimer G۲-C۵۹۵ induced no toxicity in the HEK-۲۹۳ cells and heart, liver, and brain tissues of mice. In addition, the Gd(III)-dendrimer G۲-C۵۹۵ showed specific anti-action against the in-vivo tumor model. Therefore, the Gd(III)-dendrimer G۲-C۵۹۵ nanoprobe is highly recommended as a novel and effective MR contrast agent and antitumor carrier agent. Furthermore, the Gd(III)-dendrimer G۲-C۵۹۵ nano-sized probes demonstrated excellent biocompatibility and safety with no impact on normal organ functioning.کلیدواژه ها
Gd(III)-Dendrimer G۲-C۵۹۵ Nanoprobe, Human Embryonic Kidney Cells (HEK-۲۹۳), human breast cancer cell line (MCF-۷), Nanoparticle Toxicityاطلاعات بیشتر در مورد COI
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