Probing the effects of synthesized silver nanowire/reduced graphene oxide composites on the structure and esterase-like activity of human serum albumin and its impacts on human endometrial stem cells: A new platform in nanomedicine

سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 368

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شناسه ملی سند علمی:

JR_NAMJ-8-1_005

تاریخ نمایه سازی: 6 اردیبهشت 1400

چکیده مقاله:

Objective(s): Nowadays, the unique and fascinating properties of graphene‐based nanocomposites make them one of the most promising materials for therapeutics, delivery carriers as well as tissue engineering. On the other hand, silver nanowire has been attracting more attention in nanomedicine applications, too. In this study, the effects of synthesized silver nanowire/reduced graphene oxide (AgNWs/rGO) composites on the structure and esterase-like activity of Human Serum Albumin (HSA), as well as its impacts on Human Endometrial Stem Cells (hEnSCs), were evaluatedMaterials and Methods: AgNWs/rGO composite was first synthesized and fabricated. Subsequently, its effects on the structure and esterase-like activity of HSA were evaluated by UV-Visible spectroscopy, circular dichroism spectroscopy, and fluorescence spectroscopy. Afterward, its impacts on the viability and growth of hEnSCs were studied by MTT assay, DAPI staining, and flow cytometry analysis.Results: The spectroscopic results showed that AgNWs/rGO composite could form a complex with HSA, however, did not affect the secondary structure of HSA and the binding constant for this complex was found to be ۵.۴×۱۰۴ mL.mg-۱. Furthermore, HSA maintained most of its activity in the presence of the AgNWs/rGO composite. Based on FRET (fluorescence resonance energy transfer) data the value of r۰ was less than ۷ nm signifying that the energy transfer from HSA to AgNWs/rGO composite occurs with a high level of possibility. The MTT assay, DAPI staining, and flow cytometry analysis indicated that the AgNWs/rGO composite was non-toxic towards hEnSCs. Conclusion: Our results suggest that the prepared AgNWs/rGO composite, potentially, is suitable in nanomedicine applications such as tissue engineering and drug delivery.

کلیدواژه ها:

Esterase-Like Activity ، Human Endometrial Stem Cells ، Human Serum Albumin (HSA) ، Silver nanowire/reduced graphene oxide ، Zeta Potential

نویسندگان

Azadeh Hekmat

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Shadie Hatamie

Institute of NanoEngineering and MicroSystems, National Tsing Hua University, Hsinchu ۳۰۰۱۳, Taiwan

Elham Bakhshi

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

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  • 1.Shin SR, Li Y-C, Jang HL, Khoshakhlagh P, Akbari M, ...
  • 2.Roy I, Rana D, Sarkar G, Bhattacharyya A, Saha NR, ...
  • 3.Yang K, Feng L, Shi X, Liu Z. Nano-graphene in ...
  • 4.Chung C, Kim Y-K, Shin D, Ryoo S-R, Hong BH, ...
  • 5.Hatamie S, Ahadian MM, Zomorod MS, Torabi S, Babaie A, ...
  • 6.Hatamie S, Mohamadyar-Toupkanlou F, Mirzaei S, Ahadian MM, Hosseinzadeh S, ...
  • 7.Nazari H, Azadi S, Hatamie S, Zomorrod MS, Ashtari K, ...
  • 8.Hatamie S, Akhavan O, Sadrnezhaad SK, Ahadian MM, Shirolkar MM, ...
  • 9.Yang K, Wan J, Zhang S, Zhang Y, Lee S, ...
  • 10.He Y, Jiao B, Tang H. Interaction of single-stranded DNA ...
  • 11.Sun B, Zhang Y, Chen W, Wang K, Zhu L. ...
  • 12.Hekmat A, Saboury AA, Divsalar A. The effects of silver ...
  • 13.Xia Y, Yang P, Sun Y, Wu Y, Mayers B, ...
  • 14.Zhang Y, Xu J, Yang Y, Sun B, Wang K, ...
  • 15.Hekmat A, Salavati F, Tackallou SH. The Effects of Paclitaxel ...
  • 16.Liu Z, Chen X. Simple bioconjugate chemistry serves great clinical ...
  • 17.Purcell M, Neault JF, Tajmir-Riahi HA. Interaction of taxol with ...
  • 18.Mohamadi F, Ebrahimi-Barough S, Nourani MR, Mansoori K, Salehi M, ...
  • 19.Mobarakeh ZT, Ai J, Yazdani F, Sorkhabadi SMR, Ghanbari Z, ...
  • 20.Zhang Y, Xu J, Yang Y, Sun B, Wang K, ...
  • 21.Zhang L, Zhu W, Huang Y, Qi S. Synergetic Effects ...
  • 22.Saini P, Sharma R, Chadha N. Determination of defect density, ...
  • 23.Maurya N, Maurya JK, Singh UK, Dohare R, Zafaryab M, ...
  • 24.Tran QT, Huynh TMH, Tong DT, Nguyen ND. Synthesis and ...
  • 25.Fallahi H, Azizi H, Ghasemi I, Karrabi M. Preparation and ...
  • 26.Yousef S, Mohamed A, Tatariants M. Mass production of graphene ...
  • 27.Wang Y, Wang X, Wang J, Zhao Y, He W, ...
  • 28.Amani N, Saberi MR, Khan Chamani J. Investigation by fluorescence ...
  • 29.Chaves OA, Amorim APdO, Castro LH, Sant’Anna CMR, De Oliveira ...
  • 30.Nan Z, Hao C, Ye X, Feng Y, Sun R. ...
  • 31.Zhang H, Liu W, Yang L, Liu J, Wang Y, ...
  • 32.Ranjbar S, Shokoohinia Y, Ghobadi S, Bijari N, Gholamzadeh S, ...
  • 33.Hekmat A, Saboury AA, Divsalar A, Seyedarabi A. Structural effects ...
  • 34.Zhao X, Liu R, Chi Z, Teng Y, Qin P. ...
  • 35.Masters B. Principles of Fluorescence Spectroscopy, Third Edition. J Biomed ...
  • 36.Lu Q, Chen C, Zhao S, Ge F, Liu D. ...
  • 37.Luo H, Song C, Lin X, Peng Z, Weng L, ...
  • 38.Hekmat A, Hajebrahimi Z, Motamedzade A. Structural Changes of Human ...
  • 39.Corrêa DH, Ramos CH. The use of circular dichroism spectroscopy ...
  • 40.Fu X-B, Liu D-D, Lin Y, Hu W, Mao Z-W, ...
  • 41.Ghosh D, Dey SK, Saha C. Mutation induced conformational changes ...
  • 42.Sekowski S, Kazmierczak A, Mazur J, Przybyszewska M, Zaborski M, ...
  • 43.Kubiak-Ossowska K, Jachimska B, Mulheran PA. How negatively charged proteins ...
  • 44.Lehmann SG, Toybou D, del Real A-EP, Arndt D, Tagmount ...
  • 45.An HJ, Sarkheil M, Park HS, Yu IJ, Johari SA. ...
  • 46.Wang F, Wang Y, Qu G, Yao X, Ma C, ...
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