Protective effects of Ceratonia siliqua extract on protamine gene expression, testicular function and testicular histology in doxorubicin-treated adult rats
- سال انتشار: 1399
- محل انتشار: International Journal of Reproductive BioMedicine، دوره: 18، شماره: 8
- کد COI اختصاصی: JR_IJRM-18-8_010
- زبان مقاله: انگلیسی
- تعداد مشاهده: 320
نویسندگان
Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran.
Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran.
Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
چکیده
Abstract Background: Spermatogenesis is a complex process under the influence of many different genes. Objective: This study was to investigate the possible effects of Ceratonia siliqua hydroalcoholic extract (CSHAE) on protamine gene expression, testicular function and testicular histology in doxorubicin-treated rats. Materials and Methods: 56 adult male rats (210± 10 g) were divided into 7 groups (n=8/each). The control group left untreated; the sham group received 0.3ml distilled water intraperitoneally; The negative control group received 3 mg/kg doxorubicin, intraperitoneally, once a week for 28 days; the positive control group received 600 mg/kg of CSHAE, orally, for 48 days; the experimental groups 1, 2 and 3 received 150, 300 and 600 mg/kg of CSHAE, orally, respectively for 48 days, as well as 3 mg/kg doxorubicin once a week for 28 days. Hematoxylin-eosin staining was used in histological study of testes, and enzyme-linked immunosorbent assay method was used in measuring serum levels of testosterone. Protamine gene expression was determined by Real Time PCR method. Results: Mean body weight (p=0.009), testicular weight (p< 0.001), testicular volume (p< 0.001), testosterone level (p=0.022), the count of Leydig (p=0.014), spermatogonia (p =0.046), spermatocyte (p =0.025), and spermatid (p=0.006) cells, as well as protamine gene expression (p=0.008) were significantly increased in experimental group 2 compared to the negative control group. The testicular tissue regeneration was observed in the experimental group 2. Conclusion: CSHAE has protective effect on doxorubicin-induced testicular injuries.کلیدواژه ها
Ceratonia siliqua, Doxorubicin, Protamine, Testicular injuries, Rat, سراتونیا سیلیکوا, دوکسوربیسین, پروتامین, بیضه, موش صحرایی.اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.