MGMT methylation alterations in brain cancer following organochlorine pesticides exposure

  • سال انتشار: 1399
  • محل انتشار: مجله مدیریت و مهندسی بهداشت محیط، دوره: 8، شماره: 1
  • کد COI اختصاصی: JR_EHEM-8-1_007
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 397
دانلود فایل این مقاله

نویسندگان

Fatemeh Yousefi

Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

Gholamreza Asadikaram

Corresponding author:Department of Clinical Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Saeid Karamouzian

Department of Neurosurgery, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Moslem Abolhassani

Department of Clinical Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Vahid Moazed

Department of Hematology and Oncology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Mohammad Hadi Nematollahi

Department of Clinical Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran

چکیده

Background: Alterations in the methylation levels of tumor suppressor genes are considered as one of the essential aspects of malignancies. The present study explored the association of O6-methylguanine- DNA methyltransferase (MGMT) gene promoter methylation, as a tumor suppressor, with some organochlorine pesticides (OCPs) in primary brain tumor (PBT) patients. Methods: The present study was conducted on a total of 73 PBT patients. The patients’ serum was analyzed using gas chromatography for seven OCP derivatives. The methylation-specific PCR (MSP) method was also used to determine the methylation status of the MGMT promoter. Results: The current findings demonstrated that the methylation of MGMT promoter occurred in 22 out of 34 glioma cases (64%), but in only one out of 35 meningioma cases. No MGMT promoter methylation was observed in other PBT, hemangioma, and anaplastic medulloblastoma stages. Besides, there were significant associations between MGMT methylation and γ-HCH (odds ratio [OR]: 1.50; 95% CI: 1.03- 2.40, P= 0.04), 4,4DDE (OR: 1.44; 95% CI: 1.01- 2.05, P= 0.02), 2,4 DDT (OR: 1.23; CI: 1.04- 1.45, P= 0.03), and 4,4DDT (OR: 1.46; CI: 1.23- 2.15, P= 0.02) in glioma patients. Conclusion: The results of the study suggested that the hypermethylation of the MGMT promoter in glioma patients is associated with increased OCPs in their serum, especially γ- HCH, 4,4DDE, 2,4DDT, and 4,4DDT. Moreover, it may lead to the hypermethylation of the MGMT promoter gene. Hence, it can be concluded that exposure to OCPs may potentially induce glioma.

کلیدواژه ها

Organochlorine, Pesticides, MGMT, DNA Methylation, Glioma

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.