Preparation of Chitosan - Glutamic acid Nanoparticles in Drug Release of Curcumin

Curcumin as the active compound has antioxidative, anti_ inflammatory, antimicrobial and anticancer properties among others. However, its disadvantageous properties like low solubility, poor bioavailability under neutral or acidic pH conditions or when exposed to light limit its clinical application. These problems can be solved by preparing nanoparticles. The Present study deals with the chemical modification of chitosan by using click chemistry technique. We were prepared the poly(L-glutamic acid) (PLGA) and chitosan (CS) microcapsules and to study the release rate of hydrophobic curcumin. The PLGA/CS microcapsules show high loading capacity and sustained release behavior. Nanoparticles morphologies were evaluated by Scanning Electron Microscopy. The drug loading and release rate of nanoparticles (PLGA/CS/ALG) were studied. Drug release rate dependence on pH and temperature. In vitro release rate was performed in acidic pH. The 50% of cells (the colorectal cell line HT-29) survived under MTT cytotoxic method after 72 hours exposed of (PLGA/CS/ALG+ CUR) nanoparticles.

کلیدواژه ها:

Chitosan ، Nanoparticles ، glutamic acid ، curcumin ، Anticancer