Isolation and Detection of Mycoplasma pneumoniae from Cell Culture by Culture and PCR
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 239
فایل این مقاله در 11 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJMM-13-3_001
تاریخ نمایه سازی: 10 اسفند 1399
چکیده مقاله:
Background: Mycoplasma pneumoniae is one of the major species of mycoplasmas which could contaminate cell cultures. Identification of
M. pneuminae is significant because Mycoplasmas could contaminate and have unsuitable effect on the cell cultures. Rapid detection of these contaminations is so important and it could be significant role in preventing and controlling of contamination in cell cultures. The aim of this study is isolation and detection of
M .pneumoniae by culture and PCR. 82 cell cultures collected and cultured in PPLO broth media supplemented for M. pnemoniae isolation.
Methods: The bacteria DNAs were extracted by phenol/chloroform method and the PCR assay amplifying the conserved region of 16S rRNA gene was applied for the detection of Mycoplasma genus in 163bp fragment and M. pnemoniae in 543bp fragment by using of MPF and MPR primers which obtained from P1 adhesin gen.
Results: Of the 82 samples 48(58.5%) yielded one of the potentially pathogenic Mycoplasmas evaluated for using Mycoplasma genus PCR as diagnostic method, and from 48 samples which were positive in genus of Mycoplasma, M. pneumoniae did not detect from those sample by using those primers and there is not any M. pnemoniae detected in this study.
Conclusion: Also results of this study indicate that PCR could be an alternative method instead of the culture because according to the results of this study, PCR has high accuracy and speed for detecting M. pneumoniae.
کلیدواژه ها:
نویسندگان
Salimeh Ahangaran
Mycoplasma Reference laboratory, Razi Vaccine and Serum Research Institute, Karaj, Iran
Seyed Ali Pourbakhsh
Mycoplasma Reference laboratory, Razi Vaccine and Serum Research Institute, Karaj, Iran
Alireza Abtin
Department of Microbiology, Islamic Azad University, Science and Research Branch, Tehran, Iran
Esmaeil Asli
Mycoplasma Reference laboratory, Razi Vaccine and Serum Research Institute, Karaj, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :