The Effect of Omega Fatty Acids on Bcl-2 Gene Expression and Protein Production in Cancerous Gastric Tissue

  • سال انتشار: 1396
  • محل انتشار: مجله تحقیقات سرطان، دوره: 1، شماره: 4
  • کد COI اختصاصی: JR_MCIJO-1-4_005
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 187
دانلود فایل این مقاله

نویسندگان

Homa Dokht Ghanbarzadeh

Department of Genetics, School of Basic Sciences, Islamic Azad University, Ahar, Iran

Mojtaba Mohaddes

Department of Medical Genetics, faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

چکیده

Introduction: Gastric cancer is the fourth common cancer and the second leading cause of death from cancer worldwide. Omega fatty acids are a group of non-saturated fatty acids of long chains derive from linolenic, linoleic and oleic fatty acids. The current study aimed at examining the possible effects of oral administration of omega-3, -6 and -9 fatty acids on gastric cancer cell apoptosis in patients with gastric adenocarcinoma via the evaluation of BCL2 gene and protein expression. Methods: In the present case-control study, a total of 34 patients with gastric cancer referring to the Endoscopy Clinic at Tabriz University of Medical Sciences were selected and divided into 2 groups (n = 17). In group 1, patients were treated by platinum drugs without any supplementation and in group 2, patients were treated by platinum drugs supplemented with omega-3, -6 and -9 fatty acids over 3 courses, which each lasted 3 weeks. After stomach biopsy, RNA extraction, cDNA synthesis and real-time polymerase chain reaction (RT-PCR) were performed. Results: The results showed that Bcl-2 gene and BCL2 protein expression in patients who received omega-3, -6 and -9 fatty acids with chemotherapy was lower than those of the ones who not received omega fatty acids. Also, there was a significant decrease in Bcl-2 gene expression (P < 0.05). Conclusions: The oral administration of omega-3, -6 and -9 fatty acids with the chemotherapeutic drug cis-platinum in patients with gastric adenocarcinoma could decrease Bcl-2 gene expression and BCL2 protein production in the cancerous gastric tissue.

کلیدواژه ها

Fatty Acids, Apoptosis, Stomach Neoplasms, Drug Resistance.

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.