The Effect of Omega Fatty Acids on Bcl-2 Gene Expression and Protein Production in Cancerous Gastric Tissue
- سال انتشار: 1396
- محل انتشار: مجله تحقیقات سرطان، دوره: 1، شماره: 4
- کد COI اختصاصی: JR_MCIJO-1-4_005
- زبان مقاله: انگلیسی
- تعداد مشاهده: 187
نویسندگان
Department of Genetics, School of Basic Sciences, Islamic Azad University, Ahar, Iran
Department of Medical Genetics, faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
چکیده
Introduction: Gastric cancer is the fourth common cancer and the second leading cause of death from cancer worldwide. Omega fatty acids are a group of non-saturated fatty acids of long chains derive from linolenic, linoleic and oleic fatty acids. The current study aimed at examining the possible effects of oral administration of omega-3, -6 and -9 fatty acids on gastric cancer cell apoptosis in patients with gastric adenocarcinoma via the evaluation of BCL2 gene and protein expression. Methods: In the present case-control study, a total of 34 patients with gastric cancer referring to the Endoscopy Clinic at Tabriz University of Medical Sciences were selected and divided into 2 groups (n = 17). In group 1, patients were treated by platinum drugs without any supplementation and in group 2, patients were treated by platinum drugs supplemented with omega-3, -6 and -9 fatty acids over 3 courses, which each lasted 3 weeks. After stomach biopsy, RNA extraction, cDNA synthesis and real-time polymerase chain reaction (RT-PCR) were performed. Results: The results showed that Bcl-2 gene and BCL2 protein expression in patients who received omega-3, -6 and -9 fatty acids with chemotherapy was lower than those of the ones who not received omega fatty acids. Also, there was a significant decrease in Bcl-2 gene expression (P < 0.05). Conclusions: The oral administration of omega-3, -6 and -9 fatty acids with the chemotherapeutic drug cis-platinum in patients with gastric adenocarcinoma could decrease Bcl-2 gene expression and BCL2 protein production in the cancerous gastric tissue.کلیدواژه ها
Fatty Acids, Apoptosis, Stomach Neoplasms, Drug Resistance.اطلاعات بیشتر در مورد COI
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