Induction of Specific Humoral Immune Response in Mice against a Pseudomonas aeruginosa Chimeric PilQ/PilA Protein
- سال انتشار: 1397
- محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 7، شماره: 1
- کد COI اختصاصی: JR_RBMB-7-1_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 296
نویسندگان
Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
Department of Medical Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran
Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran & Microbial Biotechnology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran & Microbial Biotechnology Research Center, Iran University of Medical Sciences, Tehran, Iran.
چکیده
Background: Pseudomonas aeruginosa, an opportunistic pathogen, is a common cause of healthcare-associated infections in immunocompromised individuals. The rapid emergence of multidrug-resistant strains has made P. aeruginosa infections progressively difficult to treat. In this study we evaluated the effect of a chimeric protein containing a P. aeruginosa PilQ fragment and the PilA disulfide loop (PilA-DSL) on the humoral immune response in BALB/c mice. Methods: A chimeric gene encoding an immunogenic region of PilQ and the PilA-DSL was synthesized. Following bacterial expression and purification, the protein was administered to mice and the humoral immune response analyzed. The resulting antibodies were analyzed using an opsonophagocytic killing assay. Results: The anti-recombinant protein antibody titer was significantly greater in immunized mice than in controls. In addition, antibody titers were significantly increased after booster immunizations, and the immunizations induced opsonophagocytosis of P. aeruginosa PAO1. Conclusions: These results suggest that an anti-adhesion-based vaccination may be effective in preventing P. aeruginosa infections. Further studies are needed to evaluate the abilities of such bivalent proteins to induce strong immune responses.کلیدواژه ها
Chimeric protein, PilQ, Pseudomonas aeruginosa, Type IV pili, Vaccineاطلاعات بیشتر در مورد COI
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