The in vitro Effect of Doxorubicine-G2-FA Treatment on Breast Cancer Copyright
- سال انتشار: 1399
- محل انتشار: فصلنامه طب دامی ایران، دوره: 14، شماره: 2
- کد COI اختصاصی: JR_IJVM-14-2_004
- زبان مقاله: انگلیسی
- تعداد مشاهده: 417
نویسندگان
۱Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Pasteur Institute of Iran, Research and Production Complex, Department of Research and Development
۳Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
۴Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
چکیده
BACKGROUND: To achieve delivery of a drug to tumors, folic acid (FA) was used as a targeting ligand to change nanocarriers. Since the folate receptor has more expression in several tumor types such as breast cancer. OBJECTIVES: The present study aimed to evaluate the effect of Doxorubicine-G2-FA (Dox- G2-FA) with in vitro assays. The abbreviation of G2 represents the second generation of dendrimer synthesis. METHODS: For this purpose, Dox-G2-FA was synthesized and mass spectroscopy was used to confirm the synthesized component. Also, MTT assay, flow cytometry, and gene expression assay by real-time PCR were used to evaluate cell viability, apoptosis, and necrosis. RESULTS: In this study, the effect of Dox and Dox-G2-FA on the expression of Bax, Bcl2 genes showed that there was a significant decrease in the expression of the Bcl2 gene in the Dox-G2-FA group compared to Dox and control groups( P< 0.05). Also, the results of flow-cytometry showed that apoptosis in the presence of Dox-G2-FA was greater than in the Dox group ( P< 0.05). CONCLUSIONS: Therefore, it seems that the effect of Dox-G2-FA on apoptosis is better than the effect of Dox usage alone.کلیدواژه ها
Apoptosis, Bax, Bcl2, Breast Cancer, Doxorubicine-G2-folateاطلاعات بیشتر در مورد COI
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