In silico analysis and expression of a new chimeric antigen as a vaccine candidate against cutaneous leishmaniasis
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 23، شماره: 11
سال انتشار: 1399
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 222
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شناسه ملی سند علمی:
JR_IJBMS-23-11_007
تاریخ نمایه سازی: 19 دی 1399
چکیده مقاله:
Objective(s): Since leishmaniasis is one of the health problems in many countries, the development of preventive vaccines against it is a top priority. Peptide vaccines may be a new way to fight the Leishmania infection. In this study, a silicon method was used to predict and analyze B and T cells to produce a vaccine against cutaneous leishmaniasis.Materials and Methods: Immunodominant epitope of Leishmania were selected from four TSA, LPG3, GP63, and Lmsti1 antigens and linked together using a flexible linker (SAPGTP). The antigenic and allergenic features, 2D and 3D structures, and physicochemical features of a chimeric protein were predicted. Finally, through bioinformatics methods, the mRNA structure was predicted and was produced chemically and cloned into the pLEXY-neo2 vector.Results: Results indicated, polytope had no allergenic properties, but its antigenicity was estimated to be 0.92%. The amino acids numbers, molecular weight as well as negative and positive charge residuals were estimated 390, ~41KDa, 41, and 30, respectively. The results showed that the designed polytope has 50 post-translationally modified sites. Also, the secondary structure of the protein is composed of 25.38% alpha-helix, 12.31% extended strand, and 62.31% random coil. The results of SDS-PAGE and Western blotting revealed the recombinant protein with ̴ 41 kDa. The results of Ramachandran plot showed that 96%, 2.7%, and 1.3% of amino acid residues were located in the preferred, permitted, and outlier areas, respectively. Conclusion: It is expected that the TLGL polytope will produce a cellular immune response. Therefore, the polytope could be a good candidate for an anti-leishmanial vaccine.
کلیدواژه ها:
نویسندگان
Leila Motamedpour
Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
Abdolhossein Dalimi
Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
Majid Pirestani
Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
Fatemeh Ghafarifar
Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
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