The evaluation of the microbial community of lower respiratory tracts microbiota in tuberculosis patients and healthy individuals using Metagenomics
- سال انتشار: 1399
- محل انتشار: مجله پزشکی بالینی، دوره: 7، شماره: 2
- کد COI اختصاصی: JR_RCM-7-2_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 110
نویسندگان
Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Microbiology and Virology, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Introduction: Tuberculosis is remained as global challenge which is considered as the top cause of human death in worldwide. The presence of lower respiratory tracts microflora can modulate immune response and play important role in susceptibility to TB. The aim of study was comparison of microbial diversity in lower respiratory tracts microflora of pulmonary tuberculosis patinas and healthy individuals. Methods: In this study, the raw sequences of SRR493275 and SRR493275 were retrieved from European Bioinformatics Institute online database. Then, the raw sequences were filtered by their quality (adapter contamination, low quality as well as low complexity reads) and taxonomic analyzed by online websites including Galaxy/CRS4 and KAIJU online servers. The statistical analysis was conducted to evaluate the presence of significant microbial diversity between two groups. Results: We found that microbial taxa were similar between TB and normal except Tenericutes which supplemented in microflora of pulmonary tuberculosis cases. Moreover, the abundance of bacterial genera is significantly divers between TB and healthy groups. Conclusion: There is significant diversities in the lower respiratory tracts microflora of TB and controls. Increasing the abundance of anaerobic genera in TB patients may be suppressed immune response and essential for susceptibility to active pulmonary tuberculosis.کلیدواژه ها
Mycobacterium tuberculosis, Microbiota, Metagenomics, 16S rRNAاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.