Study of cytotoxic of Glaucium Flavum Extract on HT-29 Cancer Cells

Introduction and Objectives: Glaucium flavum is a plant from Papaveraceae family; this plant is rich in alkaloidal components including Aporphine, protoberberine, and Glaucine. Recent research has also added antioxidant, anti-cancer, and antiviral properties to potential uses of the plant. Therefore, this study aimed to evaluate the effect of this extract on the cellular cytotoxicity of HT-29 cell line.Material and Methods: In an experimental study, the inhibitory effect of the extract at concentrations of 25, 50, 100, and 200 (μg/ ml) on the viability of HT-29 cells was investigated. Giemsa staining technique was used to study cell morphology, and SPSS version 17 was used for statistical analysis.Results: MTT results showed dose and time dependency of this extract with a reduction in cell viability, and the concentration of 50 μg/ml was used for the determination of IC50. The morphology of the cells was characterized by cell flaccidity with damage to the cell membrane.Conclusion: This extract can lead to cell death in the HT-29 cell line as a result of the effects of its alkaloids.

کلیدواژه ها:

Glaucium flavum ، MTT-assay ، HT-29