Sogand Aslani-Firoozabadi - Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Jamshidkhan Chamani - Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran

The objective of this study was to effect of piperine on human serum albumin (HSA) and holo transferrin nutural protein(HTF).HSA and HTF have shown potential in the delivery of anticancer drugs into primarily proliferating cancer.Piperine is an active alkaloid with an excellent spectrum of therapeutic activities such as anti-oxidant, anti-inflammatory, immunomodulatory, anti-asthmatic, anti-convulsant, anti-mutagenic, antimycobacterial, anti-amoebic and anti-cancer activities.Since piperine is highly lipophilic,we hypothesize that it could also interact with HSA and HTF through binding displacement and influence their bioavailability.The interaction of piperine with HSA and HTF was investigated in aqueous solution by fluorescence quenching.The quenching mechanism and binding characteristics of piperine with HSA and HTF were obtained from the fluorescence measurement.The alteration in protein conformational stability was determined by fluorescence quenching data.In addition, the alterations of HSA and HTF secondary structure in the presence of piperine were investigated.RLS spectra (Resonance light scattering) were recorded by simultaneously scanning the excitation and emission monochromators from 220 to 660 nm with Δλ=0 nm and synchronous fluorescence from 300 to 600 nm with Δλ=15 nm.The result from synchronouse fluorescence spectroscopy showed that micro-enviroment around Tryptophan and Tyrosine were changed.

HAS ,HTF ,Piperine ,Spectroscopic ,Synchronous fluorescence ,RLS ,Resonance light scattering