Association between genetic markers of PCOS1 region and polycystic ovarian syndrome

  • سال انتشار: 1399
  • محل انتشار: کنگره بین المللی علوم زیست پزشکی اصفهان
  • کد COI اختصاصی: ICIBS01_151
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 533
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نویسندگان

Ali Kowsari

Department of Mesenchymal Stem Cells, Academic Center for Education, Culture, and Research (ACECR), Qom Branch, Iran

Naser Kalhor

Department of Mesenchymal Stem Cells, Academic Center for Education, Culture, and Research (ACECR), Qom Branch, Iran

Faeze Davoodi

Department of Mesenchymal Stem Cells, Academic Center for Education, Culture, and Research (ACECR), Qom Branch, Iran

Leila Naserpoor

Department of Reproductive biology, Academic Center for Education, Culture, and Research (ACECR), Qom Branch, Iran

چکیده

Background: Polycystic Ovarian Syndrome (PCO) is one of the most common endocrine disorders in women. This syndrome is a complex and heterogeneous disorder with unknown reasons, but there are strong pieces of evidence for its classification in genetic disease. It has shown that the gene region called PCOS1 – located on the short arm of chromosome 19- is a proper candidate to assess the risk of PCO. In this study, we evaluated the polymorphisms of 9 STR markers (D19S865, D19S906, D19S840, D19S410, D19S212, STR2b, STR6b, STR5b ،D19S391 and D19S884) in this gene region and the risk of PCO in 110 women attending to highly specialized jihad daneshgahi infertility treatment center was studied using PCR and sequencing techniques.Methods: peripheral blood samples were taken from healthy and PCOS individuals, then primers and PCR were designed for STRs and then the samples were sequenced.Results: The obtained results of the demographic and hormonal analysis showed that the levels of estradiol, prolactin, LH and FSH and smoking were not significantly different between patients and healthy groups, but in case of AMH, family history, FBS and BMI there were a significant difference between the two groups. Examination of STRs in the genome of patients and healthy individuals also showed that only 2 of 7 studied STR markers (D19S865 and D19S212) were significantly associated with the risk of PCO.Conclusion: Regarding o the sensitivity and specificity of the PCOS1 region in the development of PCO, it can be concluded that this region is also important in the Iranian population as a key area associated with PCO. Therefore, by evaluating these regions, we can obtain an acceptable prediction of PCO.

کلیدواژه ها

PCO, STR, Genetic marker

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