Chicken chorioallantoic membrane as an effective animal modeling for bio molecular studies

سال انتشار: 1399
محل انتشار: کنگره بین المللی علوم زیست پزشکی اصفهان
کد COI اختصاصی: ICIBS01_139
زبان مقاله: انگلیسی
تعداد مشاهده: 431

نویسندگان

Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, (AREEO), Karadj, Iran

Rasoul Salehi

Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

چکیده

Introduction & Objectives: Animal models has been used for biological and medical study, but some ethical and biological aspects limit the whole animal usage for laboratory examinations, meanwhile the possibility of animal escaping during study is noticed by these researchers. This study suggests using of chicken embryo for bio molecular studies for simplicity and list cost.Materials & Methods: Fertile chicken eggs incubate on temperature of 37.7C and 60% humidity with torsion around vertical axes at lists 4 to 6 times a day, then germinal spot on the yolk sac differentiate to morula and followed by formation of a cavity of cellular mass blastocyst, Some cellular layers around the embryos make the allantoic sac and chorioallantoic membrane enclosed to yolk sac at the day of 8 to 9 post incubation. The similarity of the tissue response of chicken CAM with the mammalian models would be amazing for bimolecular and biometastatic study as well as microbial studies.In current study some fertile chicken egg was sanitized and prepared for incubation by a setter machine., After 4 days of in-cubation, the eggs were gently cleaned with a 70% ethanol solution. Using a 5-cc syringe and 18-gauge needle, 2.5 mLof albumen was extracted from the egg. By extracting the albumen, the CAM of the fertilized egg are separated from the top part of the shell, which allows for a small, 1.5-cm2window to be cut in the shell of the egg, without damaging the embryonic structures, the malignant colorectal cancer cells injected to CAM at the day of 7 and the window was then sealed using a transparent tape and the egg was placed back in the incubator until day 9 of incubation.Conclusion:The disseminated tumor cells were assessed in CAM and quantified by PCR.

کلیدواژه ها

CAM, Chick, Model, Bio molecular

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