The Effect Anticancer Characteristics of the Alcoholic Extract of Dracocephalum kotschyi in the Breast Cancer Cell Line

Introduction and Objectives: Breast cancer, the most frequently occurring cancer among women, is known as the fifth cause of death in Iran. Dracocephalum Kotschyi, which belongs to the Labiatae family, has been used in Iranian medicine for many years. There are almost one hundred different compounds in this plant namely as Terpenoids, Flavonoids, and Alkaloids which are considered as antioxidants substances. In this study, we investigated the effect of Dracocephalum Katschyi alcoholic extract on BAX genes expression level and proliferation on the SKBR3 cell line in breast cancer.Materials and Methods: From the Dracocephalum leaves and aerial parts, one hundred grams of powder was produced and was solved in 250ml ethanol. Using the MTT-method, different sample concentrations of the 10, 50, 100, and 500μg/ml were prepared. The effects of an alcoholic agent on cultured SKBR3 cell lines and BCL2 expression were investigated. RNAs were extracted using a QuantiTed Reverse TranscriptionKit and cDNA was synthesized. Next, through the use of a quantitative real-time polymerase chain reaction, the BAX expression was evaluated.Results: The experimental research on the Dracocephalum Kotschyi on cell proliferation and cell viability indicated that this plant is an anti-cancer substance. Besides, the IC50 value of SKBR3 cells in 24hrs was 102.1. Bax genes expression increased exposing this solvent and this was observed in the concentration of 500μg/ml.Conclusions: In conclusion, the promising results show that Dracocephalum Katschyi alcoholic extract can be used for producing anticancer medicines for breast cancer treatment in the future.

کلیدواژه ها:

BAX ، Breast Cancer ، Dracocephalum Kotschyi