Effects of intraction of Orexin B Antagonist on the Fatty Acid Synthase Enzymes Genes Expresstions of Milk Lipid in the Lactating Rats

سال انتشار: 1399
محل انتشار: کنگره بین المللی علوم زیست پزشکی اصفهان
کد COI اختصاصی: ICIBS01_112
زبان مقاله: انگلیسی
تعداد مشاهده: 349

نویسندگان

Department of Animal Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

Homayoun Khazali

Department of Animal Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

چکیده

Introduction and objection: Milk fat plays an important role in determining the quality of dairy products. Breast epithelial cells of lactating animals are largely active in triacylglycerol synthesis. The de novo synthesis of the FAS enzyme plays an important role and is used in the elongation stage.The Orexin B neurons were located in lateral hypothalamus (LHA). Orexin B or hypocretin 2 binds to the orexin/ hypocretin2 receptor. The aim of this study was to determin the effect of the intraction of the Orexin B antagonist on the expression of FAS gene involved in milk lipid synthesis in the mammary gland.Materials and Methodes: Rats that recieved salin and the Orexin B receptor antagonist at doses of 1, 2 and 4 μg/50μL. intraductal injection througt the teat can be accessed to the breast epithelium. After anesthesia and disinfection of the breast site, 20 microlitr of the materials with the desired doses is injected into each mammary duct use a 31 gavage ansolin needle. We remove the tissue 6 hours after the injection and get the result with Real Time PCR. Gene expression analysis was performed using SPSS software.Result: The results of Real Time PCR data showed that intraductal administered Orexin B antagonist increased FAS enzyme seminal activity compared to the control group(P< 0.05).Conclusion: The present study demonstrates the positive effect of successful injection of Orexin B antagonist JNJ on the FAS gene.

کلیدواژه ها

Orexin B, Milk, Antagonist JNJ

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