Bioinformatics study of single nucleotide polymorphism rs1800469 and hsa-miR-22-5p related to it in Acute Lymphoblastic Leukemia

Introduction & Objectives: One of the most common malignancies in Iran is leukemia. Evaluation of biomarkers of this disease is very importance. One of the genes involved in this cancer is TGFB1. Rs1800469 is located on TGFB1 and contains the A allele (dominant allele) and the G allele (SNP or allele). The aim of the present study was to investigate the association of rs1800469 with has-miR-22-5p and the role of TGFB1 in leukemia pathway.Materials & Methods: The micRo-pir was first used to identify microRNAs associated with TGFB1. Next, has-miR-22-5p was selected as one of the related microRNAs. This microRNA binds to the region of rs1800469. By confirming the role of has-miR-22-5p in leukemia through the phenomiR database, we identified the target genes from the miRWALK2.0 database and obtained the related signal pathways using the DAVID and KEGG databases.Results: According to studies, has-miR-22-5p binds with high affinity at the rs1800469. The case was that the microRNA binding energy of the SNP allele was higher and spontaneous, and was considered as the research hypothesis. In people with the hsa-miR-22-5p binding site, the SNP allele has greater binding ability and microRNA inhibitory action. According to the information obtained from the articles, has-miR-22-5p is an oncomir. So, it will increase expression in cancer. Therefore, TGFB1 gene inhibition will be increased.Conclusion: TGFB1 is down-regulated due to its inhibition and leads to cancer pathway. Consequently, this gene is a tumor suppressor in part of the cancer pathway due to inhibition by hsa-miR-22-5p. Based on the research hypothesis, it is expected that a SNP allele will be found in the primer design of rs1800469 in the in-vitro study of individuals with leukemia. It is anticipated that the presence of the SNP allele in rs1800469 may increase the risk of cancer and can be used as a prognostic factor for leukemia.

کلیدواژه ها:

leukemia ، TGFB1 ، hsa-mir-22-5p ، rs1800469