Evaluation of changes in enzymes of MDA, GPX, SOD, catalase and hepatocytes of male offspring of vitamin E treated rats

  • سال انتشار: 1399
  • محل انتشار: کنگره بین المللی علوم زیست پزشکی اصفهان
  • کد COI اختصاصی: ICIBS01_100
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 625
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نویسندگان

Sajjad Kooshki

M.Sc. Student in Cell Biology, Faculty of Biology, Damghan University

Mohamad Taghi Ghorbanian

PhD of Anatomy, Faculty of Biology, Damghan University

Iran Goudarzi

PhD of Anatomy, Faculty of Biology, Damghan University

چکیده

Objectives: Vitamin E can provide a good antioxidant, according to scientific evidence regarding the pharmacology and medicinal properties of the liver at different doses. Vitamin E can play an important role in healing liver damage, so we evaluated the extent of changes in liver antioxidant enzymes and liver tissue.materials and methods: Adult male and female Wistar rats weighing 220 20 20 g were used, divided into six experimental groups, and received oral gavage from day zero of gestation until 28 days after birth. And a number of offspring were anesthetized on day 28 after birth and liver was removed for histopathological evaluation and activity of antioxidant enzymes, MDA, GPX, SOD, catalase assay and stored at -80 ° C. Microtomes were cut to about 5 microns in thickness and stained by H&E method and studied by light microscopy (magnification 400). Data were analyzed by SPSS software at significant level (P < 0.05).Results: In this study, oxidative enzymes showed a significant decrease in MDA in vitamin E groups compared to the control group (P < 0.05) and GPX and catalase levels also increased significantly (P < 0.05). And in histopathological studies, the number of hepatocytes in the doses of 100, 200 and 400 vitamin E significantly increased compared to control, NAVE and oil groups (P < 0.05).Conclusion: Vitamin E has a beneficial effect on oxidative stress and reduces liver cell toxicity. It also reduces the damage of liver cells, lobules and hepatocytes.

کلیدواژه ها

Liver, Vitamin E, Antioxidant, Rat, Histopathology

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